本研究中，藉由金屬離子及聚電解質透過靜電作用力形成自組裝結構為模板，製備聚胺基酸交聯微胞、聚胺酸交聯中空奈米球及二氧化矽/聚胺酸複合奈米殼，此複合微胞是由聚二乙烯三胺五乙酸與胱胺高分子(poly (diethylenetriaminepentaacetic dianhydride-co-cystamine), poly(DTPA-co- cystm))、金屬離子及聚乙二醇嵌段聚賴胺酸高分子(PEG-b-poly(L-lysine), PEG-b-PLL)所組成，具有氧化還原及酸鹼雙重應答之性質。複合微胞之尺寸大小可藉由改變莫耳比例、金屬離子或是製程來調控。並以京尼平(genipin)交聯聚胺基酸複合微胞，使其在稀釋及溶液環境條件改變時，結構不易崩解。藉由控制京尼平與胺基(NH2)之比例，可得到不同交聯比例之複合微胞。聚胺基酸交聯中空奈米球可利用榖胱甘肽(glutathione, GSH)切斷雙硫鍵，移除聚二乙烯三胺五乙酸與胱胺高分子得到。另外，利用聚胺基酸交聯中空奈米球交聯複合微胞為模板沉析二氧化矽，可形成二氧化矽/聚胺基酸複合奈米殼，此材料可應用於生物醫學領域。此外，奈米複合微胞可包覆抗癌藥物阿黴素(Doxrubicin, DOX)，並藉由酸鹼值的降低或雙硫鍵的斷裂進行藥物釋放，此具酸鹼及氧化還原雙重應答之複合微胞將具有潛力成為藥物載體。

SUMMARY
In this study, we report the preparation of cross-linked polypeptide complex micelles, cross-linked polypeptide nanocages, and silica/polypeptide hybrid nanoshells templated by metal ion-polyelectrolyte assemblies via electrostatic interaction. The complex micelles composed of poly(diethylenetriaminepentaacetic dianhydride-co-cystamine) (poly(DTPA-co-cysam)), metal ions, and PEG-b-poly (L-lysine) (PEG-b-PLL) exhibited redox/pH dual-responsive property. Their sizes can be tuned by varying the molar ratio, metal ion species, or synthesis process. These complex micelles can be cross-linked by genipin to prevent dissociation upon dilution. The cross-linked polypeptide nanocages can be prepared and used as template to form silica/polypeptide hybrid nanoshells. Furthermore, these cross-linked micelles were employed for doxorubicin (DOX) encapsulation and it was found that the DOX release can be triggered by redox/pH dual-responsiveness. These complex micelles could be promising as drug carriers.
Keywords : nanocage, nanoshell, silica, redox/pH responsiveness, drug delivery, cross-linked, polypeptide

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