界面活性劑所自組裝成的似海綿狀的L3相，黏度低，且具有雙連續性的結構，因此以其良好的疏水親合性應用於萃取環境中微量存在的多苯環芳香烴化合物，期望能得到良好的萃取效果。在本研究中，使用了略疏水性界面活性劑Tergitol 15-S-5、Brij 30、和LE-203所形成的L3相，透過電解質及四至六碳醇類的添加，來萃取十種由二至五個苯環所構成的多苯環芳香烴化合物，並以搭配紫外光/可見光偵測器的液相層析儀進行分析，藉以探討其增濃及回收之效應。
　　
　　研究結果發現，影響增濃因子主要有三項因素，一是界面活性劑濃度；二是添加物種類與濃度，三是萃取溫度效應。如果要得到較高的增濃因子，必須以較少界面活性劑濃度、添加更多具有脫水效應的添加物、或是提高萃取溫度來進行萃取。本研究藉由0.2 wt% Tergitol 15-S-5以及5.6 wt%氯化鈉的添加，可以得到約200倍的增濃效果，並且更進一步驗證了L3相對於水中微量存在的多苯環芳香烴化合物有著相當高的回收效率。

　The isotropic spongelike L3 phase of slightly hydrophobic nonionic surfactants, Tergitol 15-S-5, Brij 30, and LE-203, were chosen as extractants for preconcentrating trace of ten selected polycyclic aromatic hydrocarbons (PAHs) from aqua prior to analysis by the high-performance liquid chromatography (HPLC) with a UV/Vis detector. These selected PAHs contain two to five rings and nine out of ten PAHs studied are listed as the US EPA 16-PAH. With its bicontinuous network structure, low viscosity and higher hydrophobic affinity, L3 phase is expected to be able to significantly improve the performance of the surfactant-based extraction process. Moreover, the extraction processes were carried out at 25°C with addition of proper electrolytes and alcohols.
　　
　Our experimental results show that the main factors influencing the efficiency and preconcentration factors of PAH extraction include the surfactant concentration, the additive type and concentration, as well as the difference between the extraction temperature and the phase transition temperature. Higher preconcentration factors could be achieved by lowering surfactant concentration, increasing concentration of salting-out electrolyte, and raising extraction temperature. A preconcentration factor as high as circa 200 could be obtained for selected PAHs with 0.2 wt% Tergitol 15-S-5 having 5.6 wt% NaCl added. Furthermore, recovery efficiencies near ca. 100% for these PAHs were garnered in these preconcentration processes, which has successfully demonstrated that the surfactant-based extraction and preconcentration technique using L3 phase could effectively extract PAHs from aqueous solutions.

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高櫻芬，「高屏溪河口與近岸海域沉積物中石油衍生性有機化合物及重金屬含量分析研究」，國立中山大學海洋環境及工程研究所碩士論文 (2001)