本研究嘗試以本土破囊壺菌DJ3萃取而得之藻油做為製備藥物載體的材料，期望達到藻油高值化的目標。研究內容著重於製程的開發，以及載體的物化性質、物理穩定性、藥物包覆效率等的探討，並利用小角度X光散射及穿透式電子顯微鏡分析載體的結構。結果顯示透過強制型製程所得到的藻油聚集體呈球狀，由TEM影像可斷定其為液胞結構。以總濃度為10 mM的藻油所製備的液胞初始平均粒徑約為169 nm，界面電位為負值，並有很優異的物理穩定性。而以藻油液胞做為葉黃素的載體，可達到約43%的包覆效率。葉黃素的存在雖使得藻油液胞雙層膜結構的流動性微幅下降，但對其物理穩定性無顯著的影響，可見以藻油液胞做為葉黃素的載體具可行性。

A microalgal lipid mixture rich in DHA was extracted from an isolated Thraustochytrium sp. DJ3 strain. This study aims to apply the microalgal lipid as delivery carrier of microalgae-extracted lutein in order to find a high value utilization of the microalgal lipid. In this study, the microalgal lipid was used to fabricate bilayered vesicles with negative charges by a forced formation approach. Furthermore, the vesicles were used as carriers for encapsulating microalgae-extracted lutein. Physical properties of the vesicles, such as initial size and zeta potential, are measured. Fluidity of the vesicular membranes with and without lutein was then investigated by infrared spectroscopy. The results showed that stable vesicles with negative charges could be successfully formed from the microalgal lipid with or without lutein. Microalgal lipid vesicles remained stable after being diluted to 5 mM and 2.5 mM. The existence of lutein had no effect on the initial size and zeta potential of the vesicles but slightly decreased the membrane fluidity of the vesicles. The existence of lutein had no adverse effect on the stability of the microalgal lipid vesicles, which showed the potential of using microalgal lipid for fabricating lutein-encapsulating vesicles. It was found that the encapsulation efficiency of lutein was about 41 %.

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