本文係針對微晶片聚合酶連鎖反應器（PCR Chip），利用ANSYS模擬軟體進行分析，以探討晶片中樣本的溫度分佈情形，首先評估最佳熱電阻尺寸配置方式，並分析各元件的材料對於樣本均溫效率的影響，最後，利用最佳化方法，在合理之設計變數變化範圍內，找出具有最佳均溫效率之樣本尺寸設計。
研究結果顯示，維持熱電阻斷面積與電阻值固定的前提下，即適當調整熱電阻厚度並以長度之變化補足電阻差值，將可獲得較佳的均溫效果，此外，當熱電阻的表面積越大時，具有較佳的均溫性，其次，在各元件的材料組合方面，上蓋板與基材使用PMMA使得熱量不易散失，故形式四之組合一的均溫效率為84.1％。再者，在限定條件下，進行樣本的尺寸最佳化分析，可得最佳的樣本尺寸，長度為3054μm，寬度為2502μm，其高度為119μm，該組合之樣本均溫效率為96％。

This thesis focuses on polymerse chain reaction chip (PCR chip).By using finite element software, ANSYS ,evaluating temperature distribution in the sample of the chip. First, evaluate the best dimension arrangement of heat resistance; and then analyze the uniform temperature efficiency of the material of component . Finally
,use the method of optimal design to find out the best uniform temperature efficiency design under the reasonable ranges of design variables.
The research results demonstrate that the best heat resistor dimension arrangement concerning uniform temperature efficiency could be achieved on the premise of sustaining the cross-section area of heat resistor and resistance value ,which mean to adjust the thickness of heat resistor and compensate the difference of resistance value properly by moderating the length of heat resistor .Besides ,when the surface area is large ,the uniform temperature efficiency is better ,In addition ,in terms of material combination , using PMMA as material of cover plate and substrate can prevent thermal energy to be lost , hence the uniform temperature efficiency of set 1 of type could achieve 84.1% ,Furthermore , the best dimension of sample could be obtained by processing the optimal analysis of sample under the limited condition ,length is 3054μm ,width is 2502μm , height is 119μm ,the uniform temperature efficiency is 96% .

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