本實驗之芒蔗是台糖公司將芒草和甘蔗雜交育種而來的品種，其葉子生成快速且泛纖維素含量高達72.49%，是未來具發展潛力的生質原料。
木質纖維素是地球上蘊藏量最豐富之再生天然資源。木質纖維素可利用高濃度酸在低溫或低濃度酸在高溫水解為單醣，供後續微生物進行醱酵，但是缺點為，1.酸會對容器造成腐蝕，2.酸水解後需用大量鹼性物質調整酸鹼值，3.僅能釋放出50-60%理論值之單醣量，4.酸水解會釋放furfural、5-hydroxymethyl-furfural等不利醱酵的抑制物生成。因此，本實驗探討經前處理之芒蔗葉水解效果，以及電透析回收酸的最適化評估。本實驗提出，在固液比1:5，溫度30 oC的條件下，以4 wt%氫氧化鈉溶液浸泡芒蔗葉6小時，洗淨後的芒蔗葉浸泡在固液比1:5之5 wt%硫酸、1.2大氣壓力、溫度為121oC之條件下酸水解2小時，可得產率0.375 g/g，泛纖維素轉化率53.34 %。考量用電成本與硫酸溶液的回收，本實驗採用電透析程序，其最佳操作電壓梯度為4 V/cm、處理時間為160分鐘之環境，如此處理批次1.5公升所需能量約0.087 kWh，而硫酸回收率可達95 %以上。電透析系統可以將酸有效回收並且重複利用，可大幅降低製作還原糖所需成本。

Saccharum-Miscanthus in the present study is a hybrid of sugarcane and miscanthus from Taiwan Sugar Company. The leaves of the hybrid provide high potential as a biomass for bioenergy production due to its high containing of holocellulose and rapid growth rate.
Lignocellulose is the most abundant renewable nature source in the Earth. Hydrolysis of lignocellulose have been applied for reducing sugar production by using concentrated acid under low temperature or dilute acid under high temperature. However, the shortage are 1.acid will corrode the containers. 2. addition of large amount of alkaline solution is required to adjust pH value. 3. it only releases 50-60 % of the theoretical yield of the reducing sugar. 4. reaction of acid hydrolysis will give byproducts which are inhibitors to the following fermentation process. In this work, we investigated the hydrolysis effect of Saccharum-Miscanthus leaves on pretreatment and electrodialysis for recovery of acid. In the proposed process, Saccharum-Miscanthus leaves were first soaked in 30 oC, 4wt % NaOH solution for 6hr followed by 5 wt% H2SO4 solution hydrolysis for under 121 oC, 1.2 atm for 2 hr. The total reducing sugar yield was 0.375 g/g raw material, conversion of holocellulose was 53.34 %. We evaluated the performance of the electrodialysis process by taking electricity consumption and recovery of acid for reusage into account. The experiment results show that the removal efficiency of separation reaches up to 95 % at the optimal conditions of applied voltage gradient 4 V/cm for 160 min, and the energy consumption is approximately 0.087 kWh for 1.5 L solutions. Electrodialysis could recover acid effectively and reduce operation cost for producing reducing sugar.

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