神經系統主要由神經細胞與膠細胞這兩種細胞組成，有別於神經細胞，膠細胞在分化出來後通常需要進行移動來行使它們的完整功能。果蠅眼盤內有一種膠細胞稱為地毯膠細胞，他們的細胞膜大且薄，從眼盤後端往前延伸至眼盤的一半處，分布於感光神經元與多數視覺基底膠細胞之間。先前使眼盤內地毯膠細胞凋亡的實驗暗示了地毯膠細胞對其他視覺基底膠細胞的移動有調控能力。為進一步了解地毯膠細胞調控其他膠細胞移動的機制，我藉由基因表達分析篩選出果蠅幼蟲時期表現在地毯膠細胞的基因，觀察了部份基因在眼盤內的表現位置，並且使用核酸干擾技術降低地毯膠細胞內一系列基因的表現量，發現在這些基因表現量降低的狀態下眼盤主要出現兩種突變型：(1)地毯膠細胞本身的型態受到影響，(2)其他視覺基底膠細胞的數量以及分布方式與原本不同。這些在地毯膠細胞內表現量下降而造成地毯膠細胞型態及功能改變的基因主要屬於表皮生長因子受體傳遞路徑與細胞黏合分子這兩大類。

Nervous system is made out of two major cell types, neuron and glial cells. Different from neurons, glial cells generally leave their place of birth and migrate to positions where they can exercise their full function. One of the retinal basal glia cells (RBG) is carpet glia. There are only two carpet glia in one eye disc whose cell membrane are wide and flat and they extend their membrane anteriorly. They are thought to regulate migration of other glia cells in eye disc because ablating carpet glia results in overmigration of other RBG. In order to study how carpet glia interact with others RBG, I selected some genes from the expression profile of carpet glia and observed theirs expression in eye disc. Then I knocked down the expression of some genes in carpet glia by RNAi. By down-regulating of these genes in carpet glia, I found two main mutant phenotypes: 1) morphology of carpet glia is affected, 2) cell number of retinal basal glia is reduced in the eye disc. These knock-down genes which cause the above phenotypes are components of growth factor receptor signaling pathway and cell adhesion molecules.

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