本研究首先製備DeTMA-DS ( decyltrimethylammonium- dodecylsulfate )和DeTMA-TS (decyltrimethylammonium-tetradecylsulfate) 兩種陰陽離子界面活性劑（catanionic surfactant），連同PL-90磷脂質分別做為兩種液胞的材料。應用乙醇的共溶劑效應，採用半自發製程，製備出穩定的陰陽體(catansomes)與乙醇體(ethosomes)。本研究並進一步探討乙醇、膽固醇、液胞材料的濃度對其粒徑、界面電位、物理穩定性的影響。此外，亦以螢光劑CF (5[6] -carboxyfluorescein)做為探測分子，探討陰陽體與乙醇體的包覆效率與釋放行為。
實驗結果顯示，在緩衝溶液(tris buffer)的環境中，陰陽體較乙醇體有更佳的穩定性。添加適當濃度的乙醇可以有效促進乙醇體與陰陽體的物理穩定性，在20%乙醇濃度下有一最佳值。而膽固醇的添加可以更進一步的提昇其穩定性。在包覆行為的研究上，乙醇體的包覆效率與其粒徑有一正比關係，其攜帶螢光劑CF的方式是以「包覆」為主；陰陽體的包覆效率與膽固醇的含量有一正比關係，其攜帶螢光劑的方式除了「包覆」以外，還有「吸附」 。因此，在本研究中陰陽體的包覆效率可高出乙醇體約4倍以上。在釋放行為的研究上，透過乙醇與膽固醇的濃度可調控釋放速率，乙醇體的釋放半衰期約從一小時至一千小時，陰陽體的釋放半衰期可從數分鐘至數千小時。陰陽體在物理穩定性、包覆效率、控制釋放上的能力上都優於乙醇體，未來更可期待陰陽體成為實用的新型的藥物傳輸載體。

In a preliminary work, the stable catansomes and ethosomes were prepared from catanionic surfactant (DeTMA-DS , DeTMA-TS), and PL-90 respectively, in the presence of ethanol by the semispontaneous process. A systematic study effects of ethanol, cholesterol and vesicle materials concentrations on the size, zeta-potential, and physical stability. Furthermore, the catansomes and ethosomes were used to encapsulate 5(6) -carboxyfluorescein (CF) in order to investigate the encapsulation and release behavior of catansomes and ethosomes.
The experimental results suggest catansomes have better physical stability than ethosomes in the buffer solutions, and a optimal ethanol and cholesterol concentration was found to enhance stability of vesicles. The encapsulation efficiency of ethosomes is proportional to size, indicating that the way of ethosomes to carry drugs is through “encapsulation” ; when it comes to catansomes, the encapsulation efficiency is proportional to cholesterol concentrations, indicating the way of cantansomes to carry drugs probably include “encapsulation” and “adsorption”. The release behavior of catansomes shows a controllable wide-ranging release rate by means of ethanol and cholesterol additions.

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