目的：植物精油近年來在台灣社會漸受歡迎，芳香療法認為杜松及佛手柑精油可輔助減緩焦慮及成癮行為，但缺乏科學證據支持。本研究以動物模式研究杜松及佛手柑精油對焦慮及甲基安非他命強化作用的調節作用。
方法：以BALB/c老鼠，腹腔內或皮下注射杜松精油(1.8g/kg)後，測量其: (１) 於開放場地之活動量；　(２)於高腳十字迷宮(焦慮之動物模式)的開放臂所停留之時間及次數；　(３)於甲基安非他命引發之場地偏好(成癮行為的動物模式)實驗中的場地偏好分數。與對照組以 one-way ANOVA 進行比較。
結果：杜松及佛手柑精油組老鼠停留在開放臂的時間多於對照組，顯示其具有抗焦慮作用。訓練前急性注射杜松（腹腔內或皮下）及佛手柑（腹腔內）精油可減弱甲基安非他命引發之場地偏好。但是，訓練前10天每天皮下注射杜松及佛手柑精油無法改變甲基安非他命引發之場地偏好。此二種精油對老鼠在開放場地之活動量並無影響。
結論：在不影響活動量的劑量下，腹腔內或皮下注射杜松及佛手柑精油有抗焦慮作用，且訓練前急性注射杜松（腹腔內或皮下）及佛手柑（腹腔內）精油可減弱甲基安非他命引發之場地偏好。

The effects of juniper and bergamot essential oils (EOs) on modulating naïve anxiety responses in the elevated plus-maze (EPM) paradigm and psychostimulant-associated reinforcing efficacy in methamphetamine (MA)-induced conditioned place preference (CPP) paradigm were investigated in the current study. We found that the time spent in the open arms was higher in the juniper- and bergamot-treated groups as compared to it in vehicle-treated group, suggesting the potentially anxiolytic effects of these two EOs. Acute injections with juniper EO (1.8 g/kg, i.p., s.c.) or bergamot EO (1.8 g/kg, i.p.) before the conditioning attenuated the acquisition of MA-induced CPP, while chronic pretreatment with either EO (1.8 g/kg, s.c.) did not attenuate the acquisition of MA-induced CPP. Acute administration of both EOs did not seem to affect mouse locomotor activity. Taken together, we conclude that both juniper and bergamot EOs may exhibit anxiolytic effects and attenuate the reinforcing efficacy of MA in a dose not affecting the locomotor activity.

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