成功大學博碩士論文系統

簡易檢索 / 詳目顯示

回結果列表

研究生：	張正中 Chuang, Cheng-Chung
論文名稱：	鈦合金動物實驗研究 Animals Study of Titanium Alloys
指導教授：	朱建平 Ju, Chien-Ping 陳瑾惠 Chern Lin, Jiin-Huey
學位類別：	碩士 Master
系所名稱：	工學院 - 材料科學及工程學系 Department of Materials Science and Engineering
論文出版年：	2004
畢業學年度：	92
語文別：	中文
論文頁數：	83
中文關鍵詞：	動物實驗、鈦合金
外文關鍵詞：	titanium alloys, implants
相關次數：	點閱：57 下載：2
分享至:	分享至facebook 分享至twitter

查詢本校圖書館目錄查詢臺灣博碩士論文知識加值系統勘誤回報

　　本研究目的在於使用本實驗室開發出新型的鈦合金植入材，進行動物實驗的研究，比較一般商用的鈦合金植入材Ti-6Al-4V。評估的方法將動物實驗後的試樣，除了以掃描式電子顯微鏡觀察植入物與組織間界面的情形外，亦使用光學顯微鏡觀察植入物周圍的組織型態，同時也藉由影像軟體來計算新生骨的面積。
　　結果顯示，在相同植入週期下，Ti-Mo(II)植入材新骨生長的比例會比其他兩種植入材來得要高。然而也可證明靠近皮質骨骨內膜處的位置，容易發生骨誘導現象其活性也較高。此外，由光學顯微鏡觀察植入物周圍的組織型態，可以發現本實驗室開發出新型的鈦合金植入材Ti-Mo（I）及Ti-Mo（II）擁有良好的生物相容性，並未發現嚴重地組織發炎現象，骨整合的程度也相當地良好。

　　The purpose of this study was to use new titanium alloys implants developed in research of animals study, comparing some commercial titanium alloys implants. These implants were inserted into the femur and tibia epiphysis of rabbits and were examined after 6 , 12 and 52 weeks with optical microscope and scanning electron microscope to observe the histological morphology and bonding condition between the implant and tissue .At the same time, accounting the values of new bone by image analysis soft ware.
　　The result was that Ti-Mo(II) of titanium alloys implants performed better properties than other implants, however, osseointegration was found easily near cortical bone. In addition, we found Ti-Mo(I) and Ti-Mo(II) implants have exceptional biocompatibility and the degree of osseointegration by observation of tissue types near implants using optical microscope.

總目錄

摘要.....................................................Ⅰ
誌謝.....................................................Ⅱ
總目錄...................................................Ⅲ
表目錄...................................................Ⅳ
圖目錄...................................................Ⅴ


第一章 前言.............................................1
1-1	研究背景..........................................1
1-2	研究動機及目的....................................2

第二章 理論背景與文獻回顧..............................4
2-1	生醫材料簡介......................................4
   2-1-1 生醫材料之分類................................4
   2-1-2 金屬材料......................................7
   2-1-3 生醫用鈦合金..................................7
   2-1-4 生醫植入材須具備的性質........................8
2-2  生醫材料之生物相容性..............................9
   2-2-1 生物相容性的相關名詞..........................9
   2-2-2 生物相容性的分級.............................10
   2-2-3 生物相容性的評估方法.........................14
   2-2-4 金屬植入材的生物相容性.......................15
2-3  骨的性質與骨組織學...............................16
   2-3-1 股骨解剖學...................................16
   2-3-2 脛骨解剖學...................................17
   2-3-3 骨組織學.....................................18
   2-3-4 骨骼的生理學及理化特性.......................23
   2-3-5 骨的癒合.....................................24
2-4   文獻回顧........................................25

第三章 實驗流程與方法.................................28
3-1	實驗流程圖......................................28
   3-1-1 試片準備及處理的流程.........................29
   3-1-2 顯微鏡觀察與分析流程.........................30
3-2	實驗植入材準備與植入方法........................28
      3-2-1 植入材準備流程...............................28
      3-2-2 動物實驗.....................................33
      3-2-3 手術方法.....................................33
   
3-3   試樣準備方法.......................................36
      3-3-1 試件取樣.....................................36
      3-3-2 組織的固定...................................36
      3-3-3 脫水與滲透...................................37
      3-3-4 包埋.........................................39
      3-3-5 切片.........................................40
      3-3-6 研磨與拋光...................................41
3-4   植入物界面之觀察...................................42
      3-4-1 鍍金覆膜.....................................42
      3-4-2 掃描式電子顯微鏡（SEM）之觀察................42
3-5   組織學觀察與影像處理...............................43
      3-5-1 染色.........................................43
         3-5-1-1 H&E染色.................................43
         3-5-1-2 甲苯胺藍染色............................45
         3-5-1-3 Von Kossa 染色..........................46
      3-5-2 光學顯微鏡之觀察.............................46
      3-5-3 影像處理與骨面積之計算.......................47

第四章 結果與討論
    4-1 試樣準備之結果...................................49
    4-2 SEM界面之觀察結果................................49
    4-3 影像處理與骨面積之計算結果.......................51
       4-3-1 不同切片位置與新生骨面積之比較..............51
       4-3-2 不同植入週數與新生骨面積之比較..............52
    4-4 組織學觀察.......................................58
       4-4-1 表面組織形態................................58
          4-4-1-1 甲苯胺藍染色結果.......................58
          4-4-1-2 H & E 染色結果.........................62
          4-4-1-3 Von Kossa染色結果......................66
       4-4-2 骨傳導的現象................................68
    4-5 生物相容性之探討.................................68
       4-5-1鈦合金之骨誘導現象...........................69
       4-5-2 骨整合與骨傳導..............................69
    4-6實驗試樣準備之檢討...............................70
       4-6-1滲膠與包埋..................................71
       4-6-2切片與研磨..................................71
       4-6-3 脫膠.......................................72
  第五章 結論...........................................73
  第六章 參考文獻.......................................74



                                    

參考文獻

1. Clemson Advisory Board for Biomaterials “Definition of the word biomaterial”, The 6th Annal Intermalionel Biomaterial Symposium, April 20-24, 1974.
2. Long M, Rack HJ. Titanium alloys in total joint replacement materials science perspective. Biomaterials 1998; 19: 1621-39.
3. Rao S, Ushida T, Tateishi T, Okazaki Y, Asao S. Effect of Ti, Al, and V ions on the relative growth rate of fibroblasts (L929) . Bimed Mater Engng 1996;6:79-86.
4. Walker PR, LeBlanc J, Sikorska M. Effects of aluminum and other cations on the structure of brain and liver chromatin. Biochemistry 1989; 28: 3911-15.
5. Yumoto S, Ohashi H, Nagai H, Kakimi S, Ogawa Y, Iwata Y, Ishii K. Aluminum neurotoxicity in the rat brain. Int J of PIXE, World Science Publishing Company, 1992:2(4):493-504.
6. Steinemann SG. Corrosion of titanium and titanium alloys for surgical implants. Ti’84 Science and Technology. Ljering G, Zwicker U, Bunk editor. 1984:1373.
7. Laing, PG, Fergosun AB Jr, Hodge ES. Tissue reaction in rabbit muscle exposed to metallic implants. J Biomed Mater Res 1967;1:135-49
8. K.H. Rateitschak and H.F. Wolf, Color Atlas of Dental Medicine. Thieme Medical Publishers. 1995:11-24.
9. M.S. Block, J.N. Kent, and L.S. Guerra, Implants in dentistry.1997: 45-62.
10. J.B. Park, Biomaterials, An Introduction. Plenum Press, New York.: 1979:4-5.
11. L.L. Hench and E.C. Ethridge, Biomaterials, An Interficial Approach. Academic press, New York.1982: 18-86.
12. S.E. Lynch, R.J. Genco, and R.E. Marx, Tissue engineering:applications in maxillofacial surgery and periodontics. Quintessence Publishing Co,Inc.1999: 84.
13. G.K.H. Fallschuessel,Enssoale impalntate. In Fallschuessel G.K.H. (ed):Zahnarztliche Implantologie.1986.
14. M. Weinlaender, Bone growth around dental implants. Dent Clin North Am.35(3). 1991: 585-601
15. D.F. Williams and R. Roaf, Implants in Surgery. W.B. Saunders Company, London. 1973:149-64
16. J.D. Winter and J.L. Leray, Evaluation of Biomaterials. John Wiley & Sons, Inc.1980: 289-93
17. 楊昌輝，圖解家畜比較解剖學(上冊) 徐氏基金會出版 1978: p.76~78.
18. 陶錫珍，比較解剖實驗指導第五冊兔之解剖狀元出版社 1980 R-21、R-22、p.20~22.
19. 鄭俊達，骨科學原理及應用(上冊) 國立編譯館主編 1986 p.33-48
20. L.C. Gerstenfeld and D. Zurakowski,”Variable Hormone Responsiveness of Osteoblast Populations Isolated at Different Stage of Embrygenesis and Its Relationship to the Osteogenic Lineage” Endocrinology 137.1996: 3957-68.
21. 謝來安，家畜組織學睿熤出版社 1990 p.253-63.
22. L. Sennerby , P. Thomsen and L. E. Ericson,”Ultrastructure of the bone-titanium interface in rabbits” Journal of Materials Science: Materials in Medicine 3. 1992: 262-271.
23. L. Sennerby , P. Thomsen , L. E. Ericson , V. Tekholm and P. Astranc, Clin. Oral. Impl. Res. 2. 1991: 103.
24. L. Sennerby , P. Thomsen , L. E. Ericson , Int. J. Oral. Maxillofac. Impl. 7. 1992: 62.
25. Piattelli, A. Scarano and M. Piattelli ,”Detection of alkaline and acid phosphatases around titanium implant: a light microscopical and histochemical study in rabbits”, Biomaterials 16.1995: 1333-1338.
26. Ungersbock, S. M. Perren ,”Comparision of the tissue reaction to implants made of a beta titanium alloy and pure titanium. Experimental study on rabbits”, Journal of Materials Science: Materials in Medicine 5. 1994: 788-792.
27. K. Roser, C. B. Johansson, K. Donath and T. Albrektsson ,”A new approach to demonstrate cellular activity in bone formation adjacent to implants” , J. Biomed. Mater. Res. 2000: 281-291.
28. T. Albrektsson, A. Erikssona and M. Jacobsson,”Bone repair in implant models: A review with emphasis on the harvest chamber for bone regeneration studies”, J. Oral. Maxillofac. Impl. 4. 1989: 45-54.
29. B. Johansson, K. Roser and T. Albrektsson ,”Qualitative and quantitative observations of bone tissue reaction to anodized implants”, Biomaterials 23. 2002: 1809-1817.
30. 張仕章，病理切片技術徐氏基金會出版 1979 p.68-75.
31. R.G. Erben ,”Embedding of bone samples in methylmethacrylate:An improved method suitable for bone histomorphometry, histochemistry ,and immunohistochemistry” , J Histochem Cytochem 45.1997: 307-313.
32. M. Hahn ,H.Welkerling, G. Delling ,”Enzyme and immunohistochemistry on undecalcified bone and bone marrow biopsies after embedding in plastic: a new embedding method for routine application”. Virchows Arch [A] 420:17-24.
33. 張喜寧、夏鎮洋，穿透式及掃描式電子顯微鏡生物技術曉園出版社 1991 p.96.
34. 張喜寧、夏鎮洋，穿透式及掃描式電子顯微鏡生物技術曉園出版社 1991 p.106.
35. D. Muster, Hard Tissue Repair and Replacement, Biomaterials,
North-Holland. 1992: 171-87.
37. 張東杰，組織學圖譜（atlas of histology）藝軒圖書出版社 1987
p.28-33
38. M. J. Filiaggi, N.A.Coombs, and R.M.Pilliar,”Characterization
of the interface in the plasma-sprayed HA coating/Ti-6Al-4V
implant system”.J Biomed Mater Res. 25(10) 1991:1211-29
39. J. M. Spivak, J. L.Ricci, N.C. Blumenthal,”A new caine model
to evaluate the biological response of intramedullary bone to
implant materials and surfaces. J Biomed Mater Res.24(9)
1990:1121-49
40. D. Muster, Hard Tissue Repair and Replacement, Biomaterials,
North-Holland. 1992: 171-87.
41. C. Yang,”The effect of calcium phosphate implant coating on
osteoconduction”,Oral Surg. 92(6) 2001:606-9.
42. C. Yang,”Effect of calcium phosphate surface coating on bone
ingrowth onto porous-surfaced titanium alloy implants in rabbit
tibiae”.J Oral Maxillofac Surg.60(4) 2002:422-5.
43. J. Black, Orthopaedic biomaterials,In Research and Practice,New
York. 1988:133-212.

校內：2007-07-29公開
校外：2007-07-29公開

簡易檢索 / 詳目顯示

相關論文