鈦金屬具有良好的機械性質與生物相容性，廣泛應用於外科植體並與骨頭親密結合。透過表面改質的方式改變表面結構、表面化學性質與表面能以改變對細胞影響，藉此來改善鈦本身生物惰性而無法提高骨整合效率的缺點。多數學者分別探討陽極氧化法製備二氧化鈦奈米管與藉由末端官能基改變材料表面化性對細胞影響。但少數學者同時探討表面形貌改變結合不同表面化性與細胞間的相互關係。本實驗以二氧化鈦奈米管，利用電漿處理改善表面活性，來增加與自組裝單層膜的改質能力，透過不同功能性末端官能基的影響與有序列的二氧化鈦奈米管陣列的結合來了解其對類骨母細胞MG63之行為。
關於二氧化鈦奈米管的生成，是藉由陽極氧化的方式並選用含氟離子的電解液達到控制鈦或鈦合金生成有秩序自組裝的二氧化鈦奈米管，並且藉由浸泡的方式做為自組裝單分子膜的改質。之後將利用SEM來觀察基材的表面形態，並透過ESCA分析表面元素與鍵結情況來判定分子膜的接枝狀況，其中，可利用簡單的接觸角量測，了解材基材表面概略性評估。之後進行細胞實驗，觀察細胞在同時受奈米管與表面官能基之影響會有怎樣的行為表現，並以細胞型態、細胞貼附、增長、鹼性磷酸酶(ALP)活性觀察之。
接觸角測試與化學成分分析確認自組裝單分子膜有效改質二氧化鈦奈米管，不同官能基造成烷基>胺基>羧基之親疏水性變化以及元素磷與元素氮含量變化確認化性接枝。細胞實驗中，除了同時進行陽極氧化同時改質的表面化性的表面比僅進行陽極氧化處理的表面有更好的細胞表現，重要的是發現即使在相同的末端官能基表面，因結合不同的表面結構，細胞表現也不盡相同。

Owing to high mechanical strength and biocompatibility, titanium and its alloys are widespread used in bone and dental implant. However, titanium lacks in rapid osseointegration. It is well-know that modification of topography, chemistry, and surface energies could lead to strong impact on different cellular responses of titanium. The TiO2 nanotube arrays by electrochemical anodization has proven to change cellular recognition conformation. The chemical properties of the outer surface by changing the group attached to the tail, the surface functionalization is performed using self-assembled monolayer (SAM).
The aim of this study is integrating effects of self-assembled monolayer (SAM) modified TiO2 nanotubes on the human osteoblastic like MG63 cells response by simultaneous modulated the ordered nano-architecture and the different functional tail groups.
The formation of TiO2 nanotube was prepared by anodization method in a fluoride-containing electrolyte, and SAM surface covered with terminal methyl (SAM-CH3), amino group (SAM-NH2), or carboxyl (SAM-COOH) on a disk was prepared by simple immersion a TiO2 nanotube disk into methanol solution of corresponding commercially available alkanephosphonic acid derivatives for 24 h at room temperature. Characterization of the morphology, chemical composition, and interfacial properties of functionalized TiO2 nanotube surface was performed by scanning electron microscopy (SEM), Electron Spectroscopy for Chemical Analysis (ESCA), and contact angle (CA) measurements, respectively. Cells are cultured on specimens in vitro, and the morphology, cell adhesion, proliferation and alkaline phosphatase assay were evaluated as the index of biocompatibility.
The SAM combining the nanotube surface of was preliminary checked by contact angle of specimens with various functional groups. The result of ESCA assay could identify the success of the SAMs grafting on the nanotube surface. In vitro test, cell attachment, cell proliferation and differentiation of TiO2 nanotubes could be changed by different SAM grafting.

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