本實驗主要是探討DPPA/DPPC混合系統於氣/液界面上單分子層之行為及其沈積現象，並進而探討DPPA單分子層與副相中葡萄糖氧化酵素(GOx)的結合現象。由熱力學分析得知，DPPA/DPPC 混合單分子膜在純水或含有鈣離子的副相下，有良好的相容性，且混合單分子層比個別純成分的單分子層在熱力學上更穩定。在含有鈣離子的副相下，DPPA/DPPC 混合單分子層的極限面積與崩潰壓都較純水面上的值大，且鬆弛曲線的實驗亦發現混合系統中DPPA的含量大於60 mole%時，鈣離子的存在使單分子層的穩定性增加，沈積轉移率有明顯提升的效果，並可成功沈積多層DPPA/DPPC混合單分子膜。DPPA LB膜的 FTIR-ATR結果經過公式計算可知隨著層數的增加，其長碳鏈越偏離基板法線，薄膜排列的均勻性亦變差。GOx在DPPA單分子層上的吸附現象從表面壓-時間的吸附曲線發現二階段的平衡存在，利用等溫線及遲滯行為可知GOx吸附量在第二階段平衡時較多。由FTIR-ATR的結果得知，氣/液界面上有DPPA單分子層存在時有助於固定水溶性GOx的功能。

This study investigated the behaviors of dipalmitoylphosphatic acid (DPPA) and dipalmitoylphosphatidylcholine (DPPC) mixed monolayers at air/liquid interface, and combination behavior of DPPA monolayer with Glucose oxidase (GOx) suspended in the subphase. The miscibility of DPPA and DPPC was evaluated by the thermodynamic characteristics deduced form the pressure-area per molecule (π-A) isotherm. The results show that the two components are miscible in general on both pure water and CaCl2 solution. Y-type multilayer LB films can be prepared for DPPA and DPPA-rich monolayer at the presence of Ca+2 ions. Two equilibrium stages were found for the adsorption of GOx onto the DPPA monolayers as estimated from the variation of surface pressure with adsorption time. Accounting to the characteristic of -A isotherms and hysteresis curves, it is believed that more GOx molecules adsorbed to the DPPA monolayer when the 2nd stage equilibrium is approached compared with that of the 1st equilibrium stage. The adsorption of GOx in the DPPA monolayer is confirmed by the analysis of FTIR-ATR either for the first or second stage adsorption. On the contrary, without DPPA as adsorption template, no peak corresponding to the GOx can be found. This result indicated that DPPA has the ability to immobile GOx on substrate.

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