本論文致力於探討表面修飾及功能性包覆之硒化鎘/硫化鋅奈米量子點穩定性研究。實驗主要可分為三大方向：1、奈米量子點之製備、表面修飾及功能性包覆，首先利用化學氧化還原法合成有機相硒化鎘/硫化鋅(CdSe/ZnS)奈米量子點，接著對其表面進行親水性修飾或以高分子材料對其進行功能性包覆。2、奈米量子點穩定性的探討，實驗中藉由改變不同的溶媒、濃度、溫度、光照條件及pH值等環境控制參數，評估奈米量子點在修飾及包覆後於不同環境變因下的穩定性。3、利用於胞飲作用的生物相容性評估，實驗中分別將經表面親水性修飾及高分子材料功能性包覆的奈米量子點利用胞飲作用送入老鼠心肌細胞(H9c2 cell line)，並進一步比較兩者在細胞內的表現與差異性。偵測上則利用紫外光-可見光分光光譜儀、螢光光譜儀、螢光顯微鏡以及穿透式電子顯微鏡來對不同的奈米量子點進行定性及定量分析之比較。

研究重要成果歸類如下：首先在製程方面，藉由適當的改變製程參數，可以有效對高分子材料包覆奈米量子點的粒徑加以控制。由穩定性實驗的結果可得知奈米量子點在不同溶媒、濃度、溫度、光照條件及pH值的穩定性，可作為其後利用於細胞實驗環境的參考。首先藉由溶媒穩定性研究可發現，當利用丙酮及甲醇作為有機相奈米量子點儲存的溶媒時，奈米量子點的螢光強度在八小時內沒有衰減的情形。在濃度穩定性的研究中則發現，奈米量子點具有其最佳的濃度範圍，在有機相奈米量子點為5-10 mg/mL，而親水性奈米量子點為0.5-1 mg/mL。從溫度穩定性研究得知在4C-44C之間的溫度對奈米量子點的穩定性無重大影響。而在不同的光照條件方面，有機相奈米量子點在紫外光下曝照兩個小時，以及親水性奈米量子點曝照八個小時，都會造成嚴重的螢光淬滅效應。在pH值穩定性研究中發現，親水性奈米量子點在pH值小於7及大於10的環境下不穩定，而高分子材料包覆奈米量子點的穩定性則不受pH值的影響。最後在細胞實驗中發現，高分子材料功能性包覆奈米量子點比表面親水性修飾的奈米量子點更容易藉由胞飲作用進入細胞內，適合應用於生醫領域中作為細胞即時影像觀測。本研究的相關成果，將有助於在奈米量子點的研究上提供基礎的貢獻。

This thesis mainly focused on probing into the stability of the surface modification and polymer encapsulation of CdSe/ZnS quantum dots (QDs). The experiment was mainly divided into three parts: 1. QDs fabrication: First, we used the oxidation-reduction method to compound the CdSe/ZnS(core/shell) QDs. Second, we processed the hydrophilic modification with the QDs surface. Finally, we use the polymer material to encapsulate the QDs. 2. Discussing with the stability of QDs: We treated the different parameters to the QDs, such as various solvent, concentration, temperature, exposure, and pH value. We tried to evaluate the stability of the QDs in the different environment. 3. Biocompatible of the cell pinocytosis: We delivered the two different surface modification kinds of QDs to the H9c2 cell line and then compare with the phenomenon and difference in the cell. We have analyzed the qualitative and quantitative of the various QDs by UV-Vis spectrometer、fluorescence spectrometer、microscope and transmission electron microscope.

This research could be induced the following results: Firstly, in our fabrication process. We could control the particle size of QDs-loaded polymer by changing the formulation parameters. Furthermore, after probing into the stability of QDs, we could find that the acetone and methanol are the better solvents for QDs. The optimum concentration range of QDs in acetone was 5-10 mg/mL, and which of hydrophilic QDs was 0.5-1 mg/mL. The temperature had no apparent effect on QDs. When organic QDs were exposed to UV light for 2 hours or hydrophilic QDs for 8 hours will result in fluorescent quenching. The hydrophilic QDs were not stable in which pH value less than 7 or over than 10. Horever, the stability of QDs-loaded polymer was not effected by pH value. Finally, we found that the QDs-loaded polymer could be delivered into the cell more easily than which hydrophilic modification. Therefore, it was suitable for applying in bio-medical research area. By this study we could understand the stability and suitable reserving environment of QDs in various parameters and these would be used for advanced research.

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