氫氣已被視為21世紀的新能源，氫燃燒之後產生水，因而不會對環境造成污染為乾淨的能源。利用微生物產氫的方式已逐漸受到重視，其中利用異營菌將廢棄物分解產氫，亦可達到資源回收再利用。本研究自稻草堆肥、牛糞堆肥、稻殼和食品及酒廠廢水處理廠共分離出71株菌株，其中27株具有產氫能力，但其差異相當大。其中菌株C008為產氫活性最高的菌株，其氫轉換率為1.43mole H2/mole Glucose，仍低於目前被視為產氫能力最佳的菌株C. butyricum。分離菌株C008經由16S rDNA序列比對的結果，其親源關係與Clostridium tyrobutyricum最為相近。C008菌株屬於發酵產氫菌，在葡萄糖含Yeast extract或Peptone的複合基質其產氫量顯著的高於只含葡萄糖的單一基質。C008菌株在發酵產氫時的產物以乙酸和丁酸為主，在生長初期乙酸和丁酸的比值大於1，且在生長過程呈現二階段的產氫特性。 此外，本研究亦探討溫度、pH、Na+及K+對分離菌株C008和C. butyricum產氫的影響。

　　Hydrogen is a clean and renewable energy, not contribute to the green house effect. Bio-hydrogen production by microorganisms can be divided into two main categories: one is by photosynthetic bacteria and the other is by anaerobic fermentation. The Clostridium, has been well studies for its ability to form endospores and its potential to generate hydrogen. In this research, we focus on the indigenous Clostridia and physiological characteristics of hydrogen production. 71 strains were isolated from composts, rice shell, food and wine wastewater treatment plants, but only 27 strains can produce hydrogen. C008 strain was isolated from compost with optimum growth at 30℃ and pH 7.0. The effects of nutrients such as peptone, yeast extract, and vitamins on growth, fermentation products, and hydrogen production were discussed in this study. The enhance of hydrogen production was achieved by combination of glucose and yeast extract as the growth medium. The hydrogen production potential of Clostridium butyricum and C008 strain was 1.39 and 2.86 mole hydrogen/mole glucose, respectively. The fermentation products of Clostridium butyricum, acetate and butyrate were produced in the ratio of less than 1, however, the ratio was greater than 1 during the 20 hours growth by C008. The transition from the acidiogenic phase to solventogenic phase was not observed during the growth of C008 strain. The effects of sodium and potassium ion on the hydrogen production of Clostridium butyricum and C008 strain was also discussed in this study. The 16S rDNA-based phylogenetic analysis of C008 strain is member of Clostridia cluster I, Clostridium tyrobutyricum.

施翠盈、許恆維、劉憶芬、曾怡禎 2001 本土性梭菌屬產氫菌株之生理特性。第二六屆廢水處理技術研討會，p.1-82。［NSC-90-2211-E-006-032］

曾怡禎、許恆維、陳佳隆 2000 梭菌屬﹙Clostridium﹚的分離及其產氫活性的影響因子。第二五屆廢水處理技術研討會，p.315-320。［NSC-89-2211-E-006-032］

簡青紅、施翠盈、劉憶芬、曾怡禎 2001 本土性梭菌屬產氫菌株之分離與產氫活性。 微生物學會第35屆年會。

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