台灣白花蝴蝶蘭(Phalaenopsis aphrodite subsp. formosana)為台灣原生種蝴蝶蘭，亦是商業化育種常使用的親本。但是栽培蝴蝶蘭的生長發育過於冗長，自播種至開花可能需要2至3年栽培。為了解決栽培時間冗長的問題參閱其他文獻研究，發現在植物激素中吉貝素(Gibberellin, GA)參與的生理調控作用如，種子發芽、生長以及開花作用等正向調節。但也在後續研究中發現當 GA 訊息傳導過程中 DELLA 蛋白會扮演負調控的角色，促使 GA 無法正常傳遞導致植物的生長調控受到限制，並且在阿拉伯芥實驗中將 DELLA 蛋白過量表現會造成植物產生侏儒的外表性狀以及對於生理調控都會受到相對應的限制。因此本實驗透過中研院蘭花網站比對阿拉伯芥 DELLA 基因找出白花蝴蝶蘭之中4個 DELLA 基因，接著利用CRISPR/Cas9基因編輯技術以及農桿菌轉殖來剔除剔除白花蝴蝶蘭之中3個 DELLA 基因，結果顯示在 T0代中疑似具有1個 DELLA 基因的序列有變異，但是對於白花蝴蝶蘭的生長與發育仍然有待進一步研究。

Phalaenopsis aphrodite subsp. formosana is an endemic species in Taiwan and commonly used parental strains in breeding programs. However, the slow growth of Phalaenopsis, may take 2 to 3 years to develop from germination to flowering. Gibberellins (GA) have diverse functions in the regulation of plant growth and development such as seed germination, stem elongation and flowering. The DELLA proteins are the repressor in GA signaling transduction pathway. In Arabidopsis, overexpression of the DELLA protein could cause the semi-dwarf phenotype. In this study, three DELLA genes were identified by Blast the corresponding Arabidopsis orthologue genes against Orchidstra database. Subsequently, the CRISPR/Cas9 editing system was applied to knock out the DELLA genes in moth orchid through Agrobacteria-mediated transformation. The results showed that one of DELLA genes maybe mutated in T0 generation of transgenic moth orchid. The effects of growth and development in moth orchid resulting from the mutated DELLA gene need further to be analyzed in T1 generation.

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陳怡寬，利用細胞穿透胜肽運送 DNA 進入葉綠體，國立成功大學生物科技研究所碩士論文，2014。

陳謫凡，台灣原生種白花蝴蝶蘭細胞分裂氧化酶基因之研究，國立成功大學生物科技研究所碩士論文，2017。

黃品升，利用職務熱休克蛋白 HSP 101 來提升外源蛋白質轉譯能疫能力之研究，國立成功大學生物科技研究所碩士論文，2007。

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