在這篇論文中我們使用頻域光子遷移系統的光學方法去量測生物組織的生理參數，它是使用波長808奈米的近紅外光來量測樣本，且由於量測樣本淺層的需要，現有的擴散方程在短的偵測距離並不適合作為光傳播理論模型去精確地得知樣本的物理參數，因此我們建構快速的縮放式蒙地卡羅法作為理論模型。在這篇論文中，首先我們會先驗證我們所建構的縮放式蒙地卡羅的正確性；接著利用液態假體和固態假體的實驗來驗證利用此模型所得到的光學參數的正確性。我們利用這套系統設計了一套實驗架構來量測模擬黑色素瘤的假體；此假體乃利用矽膠製成。希望能夠經由這個實驗能夠評估腫瘤的光學性質以及其厚度。最後將這套模型用在我們所設計用來評估腫瘤性質的實驗中，計算出我們所需的物理參數，如腫瘤的吸收係數、散射係數和評估隨著腫瘤的生長厚度的變化，最後我們證明這個實驗架構在假體的研究中是可行的。

In this thesis, we will use the frequency-domain migration (FDPM) system with a 808 nm laser to quantify physical parameters of biological tissues. In order to obtain the information from superficial volumes of sample, we will conduct the measurement at a short source-detector separation. Because the diffusion theory is not valid under this situation, we establish the scaling Monte Carlo method to be a proper model that describes transportation of photons at a short source-detector separation. We will first confirm the correctness of the scaling Monte Carlo before it is used to determine the optical properties of the liquid phantoms and solid phantoms. Further, we use this system to design a measurement protocol which is employed to evaluate the melanoma phantom. The melanoma phantom is fabricated by silicone. We will determine the physical parameters such as absorption coefficient, scattering coefficient and thickness of the tumor phantoms and understand the performance of the proposed measurement protocol.

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