藍綠菌藻華為水中細胞過度繁殖的現象，除了使水質惡化外，細胞增長也常伴隨著二次代謝物的產生，這些二次代謝物可能帶來臭味、毒性的問題，增加淨水處理程序的負荷，並對用水民眾的健康造成威脅。對於藻華的應對方法，可分成(1)湖庫水體的控制以及(2)淨水流程的去除兩部分。在湖庫控制方面，常以殺藻劑或氧化劑來抑制藻體的過度生長；而在淨水流程中則常透過前氧化程序、混凝沉澱及過濾單元來去除大部分的藻體。而當處理後剩餘存活的藍綠菌，可能會受到低劑量氧化劑的刺激而產生環境壓力，隨即影響細胞的基因表現，進而改變代謝物的生成情形，若因此造成產毒量的增加，則會造成處理單元的負擔並衍生用水安全的風險。
本研究欲透過相對基因表現量、細胞破裂情形與毒素濃度生成、釋出及降解的分析，試圖以基因表現量與產毒量之間的關係模擬產毒量的變化，並以拉氏柱孢藻(Cylindrospermopsis raciborskii)為主要對象，評估H2O2無光照、UV及UV搭配H2O2的應用對於柱孢藻細胞的影響，後續可用於飲用水水源控制管理及風險評估之參考。本研究首先確立RNA樣品的保存條件，選用DNA/RNA Shield作為RNA樣品的保存藥劑，並以RT-qPCR技術(Reverse Transcription q-PCR)定量樣品中mRNA濃度以評估相對基因表現量；接著優化PMA-qPCR (Viable-PCR)應用於拉氏柱孢藻之操作條件，以30 µM的PMA染劑濃度以及5分鐘的光反應時間作為後續批次實驗的操作參數，進行細胞完整性的分析；毒素濃度則以酵素連結免疫吸附分析法(ELISA)進行分析。
上述之方法皆應用於後續之批次實驗中樣品分析，而批次實驗條件則以H2O2無光照、UV及UV/H2O2為主，分別探討H2O2、UV及‧OH對細胞造成之影響、毒素與基因表現的變化。研究結果顯示，H2O2在本研究執行的條件下，對柱孢藻毒素的降解作用可忽略不計，且在相對產毒基因表現量呈現下降的現象；在15 Wm-2 UV暴露下，柱孢藻毒素降解速率常數為9.42×10-6 s-1，柱孢藻產毒基因表現量上升；UV降解柱孢藻毒素速率明顯高於毒素的生成，柱孢藻毒素的濃度整體持平或略微下降；UV/H2O2系統則有‧OH的生成，使相對產毒基因表現量呈下降的現象，因此不會有額外的毒素增加。由於本研究執行的條件下‧OH對柱孢藻毒素的降解量可忽略不計，因此在有‧OH的條件下，柱孢藻毒素的整體趨勢仍為持平。
整體而言，以H2O2作為氧化劑在無光照環境、以及UV搭配H2O2的系統下，並不會刺激柱孢藻毒素的產生，pks相對基因表現量下降至0.2- 0.8倍，因此無衍生的柱孢藻毒素危害的風險。相對地，UV則會刺激柱孢藻細胞，使其產毒基因表現量增加至2- 4倍，導致有產生更多柱孢藻毒素的風險發生。此結果可提供後續研究或水源管理時選用氧化劑及劑量時作為參考。

Cyanobacterial blooms may deteriorate water quality, deplete dissolved oxygen and produce toxins and/or taste and odor compounds. Oxidation is a common way to control cyanobacteria in lakes, reservoirs and water treatment plants. However, if the oxidant dose is not appropriately applied, cyanobacteria cells may be only partially lysed. Under this condition, the remaining cyanobacteria may be exposed to oxidative stress, leading to changes of gene expression and toxin production. Thus, it’s important to study the responses of the cells under oxidative stress.
Cylindrospermopsis raciborskii is selected as the target species in this study, as it is one of the dominant cyanobacteria present in many reservoirs in Taiwan. Various parameters were tested in batch experiments, including light and dark condition, H2O2 in dark condition, UV, UV/H2O2 and UV/H2O2 with hydroxyl radical scavenger. The cell integrity, toxin concentration and relative gene expression were analyzed to study the cell responses under the environmental stress. For sample analysis, cell integrity was determined by PMA-qPCR, toxin concentration was detected using ELISA, and gene expression were calculated from mRNA concentration which was quantified by RNA extraction and RT-qPCR processes.
The results showed that gene expression changed in response to different environmental conditions. The relative gene expression decreased in most of batch experiments except for the low dose UV condition. The relative gene expression was up-regulated to 2- 4 fold under low dose of UV, implying relatively high risk of additional toxin production.

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