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研究生：	孫郁婷 Sun, Yu-ting
論文名稱：	含磷酸乙醇胺官能基之自我聚集性單分子層之合成、表面分析及血液相容性之研究 Synthesis, surface characterization and in vitro blood compatibility studies of the self-assembled monolayers (SAMs) containing lipid-like phosphorylethanolamine terminal group
指導教授：	林睿哲 Lin, Jui-Che
學位類別：	碩士 Master
系所名稱：	工學院 - 化學工程學系 Department of Chemical Engineering
論文出版年：	2007
畢業學年度：	95
語文別：	中文
論文頁數：	90
中文關鍵詞：	表面性質、自我聚集性單分子層、溶劑效應、血液相容性、化學分析電子光譜儀
外文關鍵詞：	blood compatibility, surface characterization, Self-assembled monolayers, ESCA, solvent effect
相關次數：	點閱：134 下載：1
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生醫材料對於生物體而言是屬於外來物質，因此表面改質、改善其生物相容性是重要的一環。自我聚集性單分子層提供一排列緻密之表面，且末端官能基的多樣性能讓我們選擇不同的官能基表面來進行表面性質以及血液相容性之探討。本研究以細胞膜中含量豐富的磷脂質結構為基礎，擬定以單一末端官能基(磷酸化乙醇胺)之硫醇，以不同溶劑及不同濃度形成之自我聚集性單分子層，進行表面性質及血液相容性之測試及探討，以了解溶劑對於單分子層結構及材料血液相容性之影響。
由靜態接觸角測試可發現，與未改質之金基材及末端為甲基之自我聚集性單分子層相比，含磷酸化乙醇胺之自我聚集性單分子層較為親水，其中又以在PBS中成長之自我聚集性單分子層最為親水。由化學分析電子光譜儀測試得到的結果推測，在表面因為立體效應及電荷效應使得排列紊亂，這也可以解釋其他條件成長之自我聚集性單分子層的接觸角稍微偏高之現象。由S2p圖譜可看出每一個條件之自我聚集性單分子層都有未鍵結的硫醇存在，這也可說明因立體效應造成排列紊亂之原因。
血液相容性測試方面，由掃瞄式電子顯微鏡影像圖發現在材料表面某些區域的血小板有極度活化、聚集的情形，此特殊的現象需更進一步的血液測試及研究探討。

For our bodies, the biomaterials are all foreign objects. This is very important that change the properties of the biomaterial surface which could be recognized as native or part of our bodies. Self-assembled monolayers have densely-packed and well-oriented characteristics. On the other hand, varying the terminal functional groups of the monolayers will let us understand the properties of the surface. This study is based on the structure of phospholipid, the most abundant component of the cell membrane. We use the self-assembled monolayers formed by single terminal group, phosphorylethanolamine, in different solvent and concentration to understand the surface characterization and blood compatibility.
According the result of static contact angle, we observe the SAMs with phosphorylethanolamine is more hydrophilic than the SAMs with methyl terminate and pure gold. The SAMs formed by PBS is the most hydrophilic one. From ESCA spectra, we infer that the SAMs structure disorder due to the steric hinder and charge effect. It also explains why the contact angles of PE SAMs are higher. The S2p spectra show us the unbond thiol existed in the SAMs structure. This is because the steric hinder.
Due to the steric hinder and charge effect of the SAMs surface, some defects exist in the SAMs structure. Platelets activate and aggregate in the region. In this case, we can not judge the blood compatibility from the result of the morphology and number of adhered platelets and need further investigation.

中文摘要……………………………………………………………..Ⅰ
英文摘要……………………………………………………………..Ⅱ
致謝…………………………………………………………………..Ⅲ
表目錄………………………………………………………………..Ⅳ
圖目錄………………………………………………………………..Ⅴ
主文
第一章	前言…………………………………………………...1
第二章	文獻回顧……………………………………………...3
2.1 自我聚集性單分子層……………………………………3
  2.2 金屬基材之影響…………………………………………4
  2.3 兩性離子官能基…………………………………………5
  2.4 溶劑對自我聚集性單分子層之影響……………………6
2.5 細胞膜的結構……………………………………………7
  2.6 凝血機制…………………………………………………8
   2.6.1 血小板之結構………………………………………...8
   2.6.2 血小板之功能………………………………………...8
   2.6.3 凝血機制……………………………………………...9
  2.7 動機與目的………………………………………………9
第三章	儀器分析及原理…………………………………….11
  3.1 物理氣相蒸鍍…………………………………………..11
  3.2 分析儀器之原理與應用………………………………..12
   3.2.1 接觸角……………………………………………….12
   3.2.2 化學分析電子光譜儀……………………………….13
   3.2.3 掃瞄式電子顯微鏡………………………………….13
第四章	實驗方法…………………………………………….15
  4.1 實驗藥品………………………………………………..15
  4.2 實驗方法………………………………………………..20
   4.2.1 合成步驟…………………………………………….20
    4.2.1.1 11-Undecylenylphosphoric Acid之合成步驟……21
    4.2.1.2 11-Undecylenylphosphoryl ethanolamine之合
成方法…………………………………………….21
    4.2.1.3 11-thioaceto-undecylphosphoryl ethanolamine
之合成方法……………………………………….22
    4.2.1.4 11-mercaptoundecanylphosphoryl ethanolamine
之合成方法……………………………………….22
   4.2.2 金基材之製備……………………………………….23
   4.2.3 自我聚集性單分子層之製備……………………….23
   4.2.4 接觸角測試………………………………………….23
   4.2.5 血小板吸附之步驟………………………………….24
第五章	結果與討論………………………………………….26
  5.1 合成之結果……………………………………………..26
   5.1.1 11-Undecylenylphosphoric Acid……………………..26
   5.1.2 11-Undecylenylphosphoryl ethanolamine……………27
   5.1.3 11-thioaceto-undecylphosphony ethanolamine……....28
   5.1.4 11-mercaptoundecanylphosphonyl ethanolamine…....29
  5.2 表面性質分析…………………………………………..30
   5.2.1 接觸角測試………………………………………….30
   5.2.2 化學分析電子光譜儀測試………………………….31
   5.2.3 血小板吸附實驗…………………………………….34
第六章	結論與未來展望…………………………………….36
  6.1 結論……………………………………………………..36
  6.2 未來展望………………………………………………..37
參考文獻…………………………………………………………….84
                                    

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