本研究以中油廢水處理廠一級溢出污水，分離出柴油分解菌株，於實驗室的50ml血清瓶石英砂中分解柴油污染物，在耗氧環境或缺氧環境以各種不同的無機營養鹽(NH4)NO3及(NH4)H2PO4生長90天的條件試驗柴油的分解速率與細菌生長量。在耗氧環境，柴油分解菌若無營養鹽時的分解率最低為12.5%，僅有氮營養鹽時的柴油分解率達到26.0%，若同時以N:P=10:1莫耳比為營養鹽其分解率可達到33.4%,另外,以Bushnell-Haas培養基當營養鹽分解率最高可達37.8 %。因此，當有氮營養鹽時的柴油分解速率可提高一倍以上。若再加入磷之營養鹽時，柴油分解速率約為無營養鹽分解之三倍。柴油分解菌若無營養鹽時，第90天的柴油分解菌生長量僅為6×106 CFU/g，以氮營養鹽時油分解菌生長量為5.9×107CFU/g。以N:P=10:1莫耳比為營養鹽時分解菌生長量為2.2 × 108 CFU/g；以Bushnell-Haas培養基當營養鹽的柴油分解菌生長量最高可達3.5×108 CFU/g。
在缺氧環境，以分解效率而言，柴油分解菌若無營養鹽時的柴油分解率最低為5.3%，僅有氮營養鹽時的柴油分解率達到7.9%，若同時以N:P=10:1莫耳比為營養鹽其分解率可達到10.9%,另外,以Bushnell-Haas培養基當營養鹽分解率最高可達14.1 %。如同耗氧環境，當有氮營養鹽時柴油分解效率可提高一倍以上。若再加入磷之營養鹽時，柴油分解速率約為無營養鹽分解之三倍。油分解菌若無營養鹽時，第90天柴油分解菌的生長量僅為 5×106 CFU/g，以氮營養鹽時的柴油分解菌生長量為3.5×107 CFU/g，以N:P=10:1莫耳比為營養鹽時的柴油分解菌的生長量為8.3×107 CFU/g，若以Bushnell-Haas培養基當營養鹽的柴油分解菌生長量最高可達1.0×108 CFU/g。因此，無論在耗氧或缺氧環境下，氮與磷之營養鹽對柴油菌分解柴油的關係影響非常重大。
將柴油分解菌株予以分離及純化，經16S rDNA基因序列分析比對主要為Pseudomonas 屬，菌種可能為Pseudomonas nitroreducens, Pseudomonas azelaica, Pseudomonas multiresinivorans 或Pseudomonas acephalitica。

The primary sewage effluents from the Waste water treatment
plant of China Petroleum Company Refinery in Kaohsiung were
isolated for bacteria that can biodegrade diesel in quartz sand contained in 50 ml of sterilized glass vials. The bacterial fluids were cultivated for 90 days of growth with the inorganic nutrients of (NH4）NO3 and （NH4）H2PO4 under the aerobic or microaerobic conditions. Then the biodegradation rates of diesel and bacterial
growth were measured. Under the aerobic conditions, the biodegradation rate of diesel without the addition of inorganic nutrients was the lowest one (12.5％ ) , but could be increased to 26.0％ when the nitrogen nutrient was added. The biodegradation rate could be increased to 33.4％ when the nutrients of nitrogen and phosphorous (10:1 by mole )and could be up to 37.8％ when the Bushnell-Haas culture medium was employed. This revealed that the biodegradation rate of diesel could be increased by one-fold when nitrogen was
added and could be increased by three-folds when nitrogen and
phosphorous were added. The diesel-biodegraded bacterial colonies after 90 days of growth without inorganic nutrients were only 6×106 CFU/g，but could be increased to 5.9×107 CFU/g when nitrogen was added and could be in creased to 2.2×108 CFU/g when the nutrients of nitrogen and phosphorous ( 10:1 by mole） and could be up to 3.5×108 CFU/g when the Bushnell-Haas cultured medium was employed.
Under the microaerobic conditions, the biodegradation rate of
diesel without the addition of inorganic nutrients was the lowest one（5.3％）, but could be increased to 7.9％ when the nitrogen nutrient was added. The biodegradation rate could be increased to 10.9％ when the nutrients of nitrogen and phosphorous (10:1 by mole） and could be up to 14.1％ when the Bushnell-Haas culture medium was employed. Similar to the aerobic conditions , the biodegradation rate of diesel could be increased by one-fold when nitrogen was added and could be increased by there-folds when nitrogen and phosphorous wore added. The diesel-biodegraded bacterial colonies after 90 days of growth without inorganic nutrients were only 5×106 CFU/g, but could be increased to 3.5×107 CFU/g when
nitrogen was added and could be increased to 8.3×107 CFU/g when nutrients of nitrogen and phosphorous（10：1 by mole）and could be up to 1.0×108 CFU/g when the Bushnell-Hass culture medium was employed. Therefore, nitrogen and phosphorous can play an important role in the biodegradation of diesel by bacteria cultured in either aerobic or anaerobic conditions. Two bacterial strains isolated from the primary sewage effluents were magnified based on 16S rRNA gene and sequenced and subsequently aligned with the reference strains. The bacterial isolated were identified as the genus of Pseudomonas, suggesting to be Pseudomonas azelaica, Pseudomonas nitroreducens, Pseudomonas multiresinivorans, or Pseudomonas acephalitica.

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