卵白蛋白(ovalbumin)是雞蛋中最主要的過敏原，在醫學研究上，卵白蛋白所引發的過敏症狀是研究過敏症狀的最主要模型。卵白蛋白存在於所有的雞蛋類製品中，且於雞胚胎中培養出的疫苗，如德國麻疹及流感疫苗中亦含有卵白蛋白。而卵白蛋白性質穩定不易去除，因此運用分子模版的特性，具有與天然抗體相似之專一性、穩定性佳、製備簡單且成本低廉，製備卵白蛋白模版，以期能有效的辨識、分離卵白蛋白，並探討未來發展成人工抗體的可能性。
　　本研究應用微熱卡計滴定結果並配合應用微接觸技術實際製備分子模版選擇最適當的功能性單體與交聯劑。經實驗證實選擇甲基丙烯酸(MAA)為功能性單體，其模印係數(imprinting factor)可達3.57，再吸附率可達73.30 ± 2.56 %。選擇聚乙二醇(400)二甲基丙烯酸酯(PEG400)為交聯劑，其imprinting factor可達4.09，再吸附率僅有15.82 ± 2.32 %。而MAA與PEG400在5 vol %與95 vol %的條件下，imprinting factor可達10.07。當先以1 mg / ml磷酸緩衝溶液(PB)的胰蛋白酶(trypsin)溶液於37 °C下3小時，再以2 wt % 十二烷基磺酸鈉(SDS) + 0.4 wt % 氫氧化鈉(NaOH) 溶液於60 °C下30分鐘為清洗條件。其移除率可達93.3 %，且再吸附率可達61.96 %，imprinting factor可達13.44。於探討模版動力吸附機制中，可求得模版上的辨識性吸附的部份，解離常數Kd 為 3.559×10-7 M，辨識性孔洞n*有4.759×10-11 mole / cm2(即模版表面的active sites); 而非辨識性吸附的部份，Kd為2.623×10-6 M，非辨識性孔洞n*有1.940×10-10 mole /cm2。
　　卵白蛋白模板在使用人血清白蛋白(HSA)、卵伴蛋白(conalbumin)、類卵黏蛋白(ovomucoid)及溶菌酶(lysozyme)在雙成份等莫爾濃度下作競爭性分析，對卵白蛋白的選擇性分別為71.79 %、93.55 %、83.4 %及89.55 %。

　　Ovalbumin is the major allergen in eggs. In medical research, ovalbumin induced allergy is a major model in studying allergy. Ovalbumin exists in all egg-produced products, and the vaccine produced from chicken also contained ovalbumin. Ovalbumin is a stable protein that is difficult to denature, so we apply the characteristic of molecular imprinting polymer, which has similar selectivity to antibody, i.e. stable, easy to fabricate and low cost. Therefore fabricating an ovalbumin imprinting polymer should be an effective way to selectivity extract ovalbumin. Additionally, we can look forward to MIPs application in immunoassays.
This research applied the Isothermal Titration Calorimeter results compared to the results of imprinted polymer using micro-contact imprinted technology to decide the optimal functional monomer and cross-linker. Using MAA as functional monomer showed that the imprinting factor can reach 3.57, the rebinding efficiency can reach 73.30 ± 2.56 %. Using PEG400 as cross-linker showed that the imprinting factor can reach 4.09, the rebinding efficiency only had 15.82 ± 2.32 %. With a ratio of MAA and PEG400 of 5 vol% and 95 vol%, the imprinting factor can reach 10.07. While using 1 mg / ml PB of trypsin solution at 37 °C for 3 hours, and then using 2 wt % SDS + 0.4 wt % NaOH solution at 60 °C for 30 minutes as extraction condition. The removing efficiency can reach 93.3 %, the rebinding efficiency can reach 61.96 % and the imprinting factor can reach 13.44. Scatchard binding plots showed the dissociation constant for the specific binding phase to be 3.559×10-7M and the theoretical recognition binding site capacity to be 4.759×10-11 mole / cm2; for the non-specific binding phase Kd =2.8086×10-6 M and the non-specific recognition binding site capacity was determined as 2.049×10-10 mole /cm2.
The selectivity binding experiments were carried out in binary protein systems with equal molecular concentrations of the proteins. In binary competition systems, ovalbumin selectivity to HSA, conalbumin, ovomucoid and lysozyme was 71.79 %, 93.55 %, 83.4 % and 89.55 % respectively.

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