本論文主要以微乳化與熱裂解兩種方法製備Fe@Au核殼型複合奈米粒子，探討產品特性，並與抗癌藥物葉酸抗拮劑(MTX)鍵結。本研究認為，若能在鐵奈米粒子的表面被覆金奈米薄層，不僅可避免鐵被氧化，且可透過表層的金與各種藥物或生物分子結合，創造出兼具磁性、光學特性、與生物或生醫功能的奈米複合體，同時達到標識、標的、及分離的目的，廣泛應用於生化與生醫領域上。

　　關於微乳化法製備Fe@Au核殼型複合奈米粒子的研究，係在water/CTAB/n-butanol/isooctane微乳化系統中進行。利用X光繞射儀、紫外光及可見光光譜儀、化學分析電子光譜儀，可證明所得產品確為Fe@Au核殼型複合奈米粒子，且無氧化鐵產生。表面經修飾後，可成功接上MTX分子。進一步探討Fe@Au核殼型複合奈米粒子對細胞的毒性，結果發現新鮮製得的Fe@Au奈米粒子相對於正常的細胞而言，對癌細胞有選擇性的毒殺效果，但經在空氣或溶液中陳化數天後，其毒性即明顯降低。由氧化測試及亞鐵離子釋放速率的研究推測，Fe@Au核殼型複合奈米粒子的鐵核表面可能透過金殼層晶粒間的微細孔隙釋出亞鐵離子，造成細胞的毒殺，但在空氣或溶液中陳化數天後，金殼層晶粒間微細孔隙內的鐵核表面，可能產生一層薄氧化層，抑制亞鐵離子的進一步釋出。據此，本研究提出一智慧型藥物的概念，利用新鮮製得的Fe@Au核殼型複合奈米粒子選擇性毒殺癌細胞，待數天後，此粒子會自身安定化，不會造成長期性的傷害。

　　關於以熱裂解法製備Fe@Au核殼型複合奈米粒子的研究，在保護劑油酸和油胺存在下，先熱裂解Fe(CO)5形成Fe奈米粒子，再利用1,2-十六烷二醇來還原醋酸金，可成功製得單分散的Fe@Au奈米粒子。由X光繞射儀與紫外光/可見光光譜儀的分析結果顯示所得產品為核殼型結構且無氧化鐵產生。

　　In this thesis, Fe@Au core-shell composite nanoparticles were prepared by microemulsion and thermal decomposition methods. The product was characterized and conjugated with the anti-cancer drug methotrexate (MTX). In the as synthesized product, the oxidation of iron nanoparticles could be hindered due to the surface coating of Au nanoshells. Also, via the conjugation with drugs or biomolecules, the product may become a nanocomposite with magnetic, optical, and biological or biomedical functions. Since the aims of labeling, targeting, and separation could be achieved simultaneously, the product may be used widely in biochemical and biomedical fields.

　　First, Fe@Au were prepared in the microemulsion system of water/CTAB/n-butanol/isooctane. By the analysis of XRD pattern, UV/VIS absorption spectra, and ESCA, the product was recognized to be the Fe@Au core-shell composite nanoparticles without the presence of iron oxide. Via surface modification, the product could be conjugated with MTX molecules. It was found that the fresh product has selective toxicity for cancer cells compared to the normal mucosal keratinocytes, while the product aged in air or aqueous solution for several days has significantly reduced toxicity . From the studies on the oxidation of the product and the releasing of ferrous ions, it was suggested that ferrous ions might release from the surface of iron core via the grain boundary of Au nanoshells. After aging in air or aqueous solution for several days, a thin layer of iron oxide might form on the surface of iron core within the grain boundary of Au nanoshells, which prevented from the further releasing of ferrous ions and oxidation of iron. Accordingly, in this study, an idea using the product as a smart drug was proposed. The fresh product could be used to kill the cancer cells selectively, while the product spontaneously transformed into nontoxic agents which significantly reduced potential accumulation of the toxic compounds and the systemic side effects.

　　Second, monodisperse Fe@Au core-shell composite nanoparticles were prepared by thermal decomposition of iron pentacarbonyl and reduction of gold acetate with 1,2-hexadecaneediol in the presence of stabilizers oleic acid and oleylamine. The analyses of XRD pattern and UV/VIS absorption spectrum revealed the formation of Fe@Au composite nanoparticles without the presence of iron oxide.

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