塗佈基材表面會改變其細胞親和性，本論文將三種塗層塗佈於鈦六鋁四釩合金表面，分別為鈣磷化合物、型一膠原蛋白及兩者之複合塗層，並評估鈦六鋁四釩合金之人類類骨細胞親和性。實驗分為四個組別，分別命名為Ti-6Al-4V、Ca-P、Collagen、Ca-P/Collagen，在塗佈試片表面之前，鈦六鋁四釩合金表面皆先進行矽砂紙的溼式研磨及氧化鋁粉的拋光程序，得到平滑的表面。Ti-6Al-4V組別不進行任何的表面塗佈程序；Ca-P組別利用浸泡模擬人體體液（simulated body fluid；SBF）的方式，於試片表面進行鈣磷化合物的塗佈；Collagen組別於試片表面進行型一膠原蛋白（type I collagen from calf skin）的塗佈；Ca-P/Collagen組別則於試片表面塗佈鈣磷化合物及型一膠原蛋白之複合塗層。在各組別試片之表面上培養人類類骨細胞（human osteosarcoma；HOS），進行細胞增殖分析以及細胞型態的觀察。細胞的新陳代謝越活躍，MTT （Methylthiazoletetrazolium）被消耗的量越多，產生的沉澱物（formazan crystal）也越多，於試片表面培養HOS細胞1、5、10天後，利用酵素免疫分析測讀儀（ELISA reader）判讀吸光度，分析細胞增殖的趨勢；於試片表面培養HOS細胞1、3、12及24小時，使用掃描式電子顯微鏡（scanning electron microscopy；SEM）觀察細胞貼附型態。透過本論文的實驗結果，比較各組別中細胞增殖的情形，發現型一膠原蛋白塗層最能助長HOS細胞的增殖，而鈣磷化合物塗層對於HOS細胞的增殖則有負面的影響；而細胞初期黏附型態方面，Collagen組別細胞的貼附最為平坦，Ca-P組別則效果最差，發現型一膠原蛋白能促進HOS細胞的初期黏附情形。

Surface modification of the substrate affects it’s cyto-compatibility. We have evaluated the HOS cytocompatibility of Ti-6Al-4V alloy after coated with Ca-P compound, type I collagen, and compound coating of both on the surface respectively. Experiment was divided into four groups, called Ti-6Al-4V, Ca-P, Collagen, Ca-P/Collagen, respectively. Before surface modification, surface of Ti-6Al-4V alloy became smooth after abrading with sandpapers and polishing with Al2O3. For Ti-6Al-4V group, sample surface coated with nothing. For Ca-P group, sample surface coated with Ca-P compound by biomimetic immersion ( simulated body fluid ; SBF ). For Collagen group, sample surface coated with type I collagen ( from calf skin ). For Ca-P/Collagen group, sample surface coated with Ca-P compound and type I collagen. After human osteosarcoma ( HOS ) cells were cultured in each groups, we evaluated cell proliferation and observed cell morphology. When cell metabolism was more active, more Methylthiazoletetrazolium ( MTT ) was wasted and more formazan crystal was appeared. After cultured for 1, 5 and 10 days, we assayed proliferation of HOS cells by ELISA reader. Cells were cultured for 1, 3, 12 and 24 hours, we observed cell morphology on sample surface by scanning electron microscopy ( SEM ). Results of experiment, we found that type I collagen was the best factor and Ca-P compound was the worst factor to enhance cell proliferation. For cell
morphology, type I collagen could help to flatten HOS cells sample surface.

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