近年來國內外漏油事件頻傳，造成嚴重的土壤及地下水油品污染，在整治方法中，生物復育因其低成本、對環境衝擊較小且可有效達到復育成果，逐漸成為土壤及地下水復育技術的趨勢。本研究以柴油為目標污染物，應用柴油降解菌於受柴油污染的土壤與地下水，本實驗分為兩部份，一為應用柴油降解菌與生物界面活性劑於離場土耕法土壤生物復育，以小型土壤反應槽模擬離場土耕法生物復育，探討額外添加柴油分解菌及生物界面活性劑對柴油降解的影響，另有一控制組作為比較，另一為應用細胞固定化技術進行地下水整治，此為未來應用於土壤管柱生物滲透性反應牆的前驅實驗，主要探討柴油降解菌在經包埋後對柴油降解的效果及其再使用性和壽命。
土壤批次實驗中，採用兩種不同菌群，皆以不同植菌量及添加生物界面活性劑與否作為實驗因子，經操作80天後，各組柴油去除率皆介於76~87％，240天可以去除90~98%柴油，而控制組在240天後僅去除78%柴油，各實驗組的第一階段降解速率常數介於-0.0185~-0.0304day-1，而控制組僅0.005day-1，經分子生物技術監測發現，實驗組在復育階段均具豐富的柴油降解菌相，反觀控制組柴油降解菌種多樣性雖有增加，但在後期卻有逐漸消失的現象，這為造成復育效果差別的主要原因，而實驗中探討的菌量多寡及是否添加20mg/kg dry soil生物界面活性劑，並沒有造成柴油降解效果的差別。
在應用細胞固定化顆粒降解水中柴油實驗裡，發現活性碳有助於顆粒內菌體的釋放，而較強顆粒強度會限制菌體釋出，而在單一菌株測試中以CC-JG39及CC-BC11具較佳的降解效果，分別為250小時殘留13%柴油及330小時殘留20%柴油，且分別具-0.0223hr-1及-0.0127hr-1第一階段降解速率常數， CC-RS1、CC-CF3及CC-DSM444-62則沒有明顯降解效果，而採用混菌在第一個批次實驗中並無明顯提升的柴油降解的現象，在經重複實驗後發現顆粒可以被重複地使用來降解柴油，且顆粒結構能保持完整、不崩解，有利於未來應用於土壤管柱模擬現地滲透性反應牆。

Oil leaking from storage tanks happens frequently, and causes serious soil and groundwater contamination. Among remediation techniques, bioremediation of petroleum hydrocarbons has been proposed as an effective, economic, and environmentally friendly technology and been applied extensively recently. This study applied diesel-degrading bacteria on diesel-contaminated soil and groundwater bioremediation and was devided into two parts. One applied bioaugmentation and biosurfactants (rhamnolipid) for enhanced ex-situ bioremediation of diesel-contaminated soil and investigated the potential application of with series of bench-scale experiments. Another applied diesel-degrading bacteria on immobilized cell technique for degrading diesel in water and investigated degradation effect and if the beads can be used repeated. We hope it can be used on permeable reactive barrier in the future.
In soil bioremediation experiments, we adopted two diesel-degrading bacteria consortias. Bacteria concentration and biosurfactant addition were two factors investigated. After 80 and 240 days, experimental groups removed 76~87% and 90~98% diesel repectively. However, control removed only 78% diesel after 240 days operation. Degradation rate constants in experimental groups were between -0.0185 day-1 and -0.0304day-1 and larger than control one -0.005 day-1. By the way, molecular methods such as microarray hybridization, T-RFLP and DGGE were used to monitor the microbial population dynamics of augmented diesel-degrading bacteria in tested bio-piles. The results showed that bioaugmentation can increase the microbial diversity of diesel-degrading bacteria and cause the diffirence between experimental groups and control. There was no significant diffirence after adding diffirent bacteria concentration and biosurfactant.
In diesel-contaminated water bioremediation, we found that active carbon can help release bacteria from gel beads to outside liquid and also found that the higher strength of gel beads will restrict bacteria move. Among five diesel-degrading bacteria, CC-JG39 and CC-BC11 had good effect to degrade diesel after immobilized. There were 13% residual diesel of CC-JG39 and 20% residual diesel of CC-BC11 after 250 and 330 hours respectively. Degradation rate constant of CC-JG39 and CC-BC11 were -0.0223hr-1 and -0.0127hr-1 respectively. CC-RS1, CC-CF3 and CC-DSM444-62 did not have good effect to degrade diesel, although CC-DSM444-62 had good diesel- degrading ability in suspended condition. When we entraped CC-JG39 and CC-BC11 individually and then mixed gel beads, mixed bacteria had no significant effect to increase degradation. After repeated batch, we found that gel beads can degrade diesel repeatedly and maintain their structure. This result gave us confidence to apply gel beads on permeable reactive barrier in the future.

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