細胞組織學上，如何判斷培養細胞的健康與行為，常使用的方法：光學顯微鏡觀察其形態上的變化、MTT分析法判斷細胞存活率。然而諸如以上各項技術，雖然可以約略窺探細胞內外資訊，但仍有其缺點：(1)無法準確量化 (2) 花費大量人力、時間 (3) 無法做即時性量測。因此本論文期望以電阻抗的量測解決上述缺失，它於生物醫學與臨床研究上是一項新的開發量測工具，其主要為利用生物不同組織既有的電特性(阻抗、導納、相角與頻率響應)所獲取的特徵響應，以及組織間的差異，做為直接量測或間接定量。目前在電阻抗分析量測上，已被廣泛應用在於生醫材料、藥物開發、環境檢測、細胞培養等各項研究與應用上。
本研究利用電阻抗系統，加上連續攝影，對細胞進行即時性電阻抗及細胞生長形態的觀測，評估細胞形態上的變化與細胞凋亡間的關聯。結果顯示細胞受到hydrogen peroxide (H2O2)的氧化傷害而產生凋亡時，會使得粒線體膜電位下降，並失去原有製造ATP的能力，此時細胞膜無法維持其內外層的對稱性，此時大量分佈於細胞膜內層的phoshpatidylserine(PS)會翻轉到外層，造成細胞電阻值明顯增加。最後加入N-acetly-L-cysteine (NAC)這類抗氧化劑後，則能使細胞免於氧化自由基造成的傷害。實驗結果將與傳統MTT、DNA fragment及流式細胞儀量測結果互相比對驗證，使得實驗數據更具公信力。

Biologically, there are a lot of methods to evaluate the condition and motion of cells, including observing morphological change of cells with microscope, assassing cell viability with MTT assay, tracing the distribution of peudopods and fluorescence. All of these methods are hard to achieve, real-time quantify and the cost is higher. An alternative on-line method to monitor cell attachment or detachment is electric impedance sensing system (EIS). This measuring method revealed several importatnt physical parameters, such as impedance(Z), admittance(R), phase angle, and frequency response.EIS is also to quantify. Recently, EIS has been applied to cell behavior quantifying , environmental monitoring, pharmaceutical screening and testing of biocompatibility.
In this thesis, we evaluated the relationship between cell apoptosisand the change of morphology in real-time by EIS. When cells startedap-optosis, the membrane potential of mitochondria decreased and ATP could not be produced. Because of balance between cell membrane inner and outer was disrupted and phoshpatidylserine(PS) in the inner layer of the cell membrane became inverted. This resulted in the change of cellular impedance. Result descried Then we tried to add an antioxidant, N-acetly-L-cysteine (NAC), to prevent cells from reactive oxygen species damage and record the change of cellular impedance again. All of these data are recorded with EIS and a series of microphotography. The result is consistent with traditional methods, MTT assay and DNA fragment.

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