石斑魚是目前台灣養殖界重要的高經濟魚種，因此藥物被濫用於促進石斑魚生長的事件時有所聞，本研究的主要目標便是安全的促進石斑魚生長以避免藥物殘留造成的危害同時提升其產業價值。由於SPARC（Secreted Protein Acidic and Rich in Cysteine）是一種細胞基質蛋白，能調節胚胎發育、細胞分化和骨骼形成以及影響生物體的脂肪生成，因此本研究以發展抑制內生性SPARC口服抑制劑為主要目標希望建立低壓迫性的免疫抑制系統，促進養殖魚隻的脂肪增長，同時增加此系統在實際養殖上使用的可能性。本研究中透過口服實驗證明利用口服SPARC抑制劑確實可誘發石斑魚免疫反應並促進石斑魚體油脂含量，同時發現實驗組石斑魚有生長較為快速的趨勢並且沒有導致石斑魚出現畸形問題，但仍不了解此實驗促進魚隻生長的原因為何，目前已知myostatin對石斑魚的肌肉生長有重要的調控功能，而文獻也指出，myostatin的訊息傳遞路徑受到follistatin進行調節，同時有多種follistatin-like的蛋白具備跟myostatin結合調控的功能，因此本研究亦已證實不論是重組蛋白還是石斑魚血清中蛋白，SPARC和myostatin都會形成複合體型態，根據此結果推測石斑魚體內SPARC可能透過和myostatin結合進行魚隻生長的調控。

In Taiwan, the grouper is an important species of aquaculture, and there are many chemical materials used to promote the fish growth, however, these materials may stay in fish body for a long period and influence the health of consumer. This research wants to improve the development of grouper farming with higher consuming value and safety. SPARC (Secreted Protein Acidic and Rich in Cystein) is a matricellular protein which can regulate embryonic development, cell proliferation, formation of skeleton and adipogenesis. In this study, we established an SPARC immuno-inhibition grouper model by oral delivery system, it can avert the stress caused by injection system and improve the possibility for real administration in aquaculture. Significant raise of anti-SPARC antibody level and weight gain found in SPARC-inhibited gropers. Increased adiposity detected and no distinct deformity found. As previous study, myostatin can control muscle growth in groupers. Interestingly, we found obvious increase of muscle growth in SPARC-inhibited groupers. Many references indicated that follinstatin-like domain of SPARC protein may have chance to bind myostatin protein and regulate the downstream signaling. Therefore, we demonstrated the interaction between SPARC and myostatin with recombinant protein and
serum by immunoprecipitation. Thus we hypothesized the increase of weight gain was caused by change of interaction between SPARC and myostatin.

粘茂偉，利用TR-PCR發展石斑魚神經壞死病毒次單位疫苗，國立成功大學生物科技研究所碩士論文，2003。

陳宛兒，點帶石斑魚肌肉生長抑制基因之功能分析及分子調控，國立成功大學生物科技研究所碩士論文，2009。

黃意菱，石斑魚活體內外之SPARC蛋白功能分析，國立成功大學生物科技研究所碩士論文，2010。

楊元智，魚類sparc基因表現及功能分析，國立成功大學生物科技研究所碩士論文，2007。

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