氧氣是植物生長的必須條件，本研究是以蛋白質的觀點探討氧氣對稻米芽鞘的影響。藉由控制氧氣的含量，讓稻米在不長根的情況下恆溫培養。等到芽鞘長到約2、3公分，將一組芽鞘通入定量空氣培養；另一組則是靜置培養，當作對照組。這樣的條件下，一直到通氣12小時都不會有長根的情形。將通氣時間不同(1、2、4、6、12和24小時)的稻米芽鞘收集起來，然後萃取芽鞘內的蛋白質。利用二維膠體電泳來研究實驗組和對照組之蛋白質，發現有新的蛋白質點出現在實驗組的二維電泳膠片中，這新的蛋白質點一為分子量約94kD在pI約為10之處，另一為分子量約14kD在pI約為3之處。LC/MS將進一步被利用來鑑定這新蛋白質點。離子交換管柱和膠體層析管柱也將被用來純化此蛋白質，再進一步研究該蛋白質在生理方面所扮演的角色(biological roles)。本研究藉由蛋白質的觀點，顯示通氣對於稻米芽鞘生長的影響。

Oxygen is requisite for plants to grow. Investigation of the effect of oxygen content on plant is then interesting. This study is to explore the effect of oxygen on the rice coleoptile in view of protein express. The rice coleoptiles were incubated in an unrooting condition by controlling the oxygen content. When the coleoptiles grew to about 2 to 3 cm, the experimental group was continued incubating with the bubbling of air, while the control group was without air bubbling. Under this condition, no roots were found for the experimental rice within 12 hours air bubbling. The coleoptiles obtained from experimental group bubbled with air for various times (1, 2, 4, 8, 12, and 24 hrs) and control group were collected and the coleoptile proteins were extracted. 2-D gel electrophoresis was employed to study the total protein differences between experimental and control coleoptile. Two additional protein spots, one with molecular weight about 94kD located at pI around 10; one with molecular weight about 14kD located at pI around 3, are found in experimental coleoptiles. These additional proteins will be further identified by LC/MS spectroscopic technique. Ion exchange and gel filtration column chromatograph also were employed to purify these proteins. It is interesting and important to further study the possible biological role for these proteins. This study has shown that air content has effects on the growth of coleoptile in terms of protein view.

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