關節軟骨因缺乏血管供應養分、細胞活性低及幹細胞數量有限，使其具較低的自我修復能力，這些特性對於骨關節炎的治療與關節軟骨再生構成重大的挑戰。在組織工程領域中，結合支架與生物活性材料被視為一項具潛力的再生策略。本研究開發了一種由去細胞化軟骨粉（DCP）與經醛基與甲基丙烯酸酯修飾的玻尿酸水凝膠（AHAMA）組成的複合材料，探討其在關節軟骨修復中的應用潛力。為評估DCP/AHAMA 是否能提供有利於細胞分化與遷移的微環境，我們進行多項體外測試，包括細胞毒性、細胞活性與細胞遷移實驗，結果顯示，該複合材料無明顯細胞毒性，並可為髕下脂肪墊間質幹細胞（IFPSCs）提供適合生長的微環境，展現良好的生物相容性。機械性質測試亦證實該複合材料具備適當的黏著性與抗壓強度。另外在三維（3D）培養實驗中，將 IFPSCs 與 DCP/AHAMA 共同培養於一般與誘導性軟骨分化培養基中，評估其軟骨分化能力，結果顯示，其軟骨分化標記(COL2 與 CAN)表現上升，而肥大分化標記(COL1)則表現下降。綜合上述，DCP/AHAMA支架可有效模擬原生軟骨的生物力學特性，亦能促進軟骨分化並抑制肥大分化，具備應用於軟骨組織工程與臨床治療之潛力。

Articular cartilage has a limited intrinsic ability to heal naturally, thus presenting a challenge for the treatment and regeneration of osteoarthritis. A potential strategy for cartilage tissue regeneration involves using scaffolds and bioactive materials to support tissue repair in degenerated cartilage. In this study, a composite material consisting of decellularized cartilage powder (DCP) and hyaluronic acid hydrogel modified by aldehyde groups and methacrylate (AHAMA) was synthesized and evaluated in vitro for its regenerative potential. Cytocompatibility assays, including cell proliferation, cell viability, and cell migration, indicated that DCP/AHAMA composite exhibits negligible or no cytotoxic effects. Additionally, it provides a supportive microenvironment for infrapatellar fat pad stem cells (IFPSCs). Mechanical testing confirmed that the composite possesses suitable adhesive and compressive strength. Three-dimensional (3D) cultures incorporating IFPSCs and DCP/AMAHA evaluated the ability of IFPSCs to differentiate into chondrocytes in both normal and chondroinductive media. Results demonstrated upregulated expression of chondrogenic markers such as COL2 and CAN, alongside downregulation of the hypertrophic marker COL1. The DCP/AHAMA scaffold effectively mimics native cartilage biomechanics, promotes chondrogenic differentiation, inhibits hypertrophic differentiation, and demonstrates promise for applications in cartilage tissue engineering and clinical treatment strategies.

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