了解細胞黏附力於生醫材料上的表現可最為評估生醫材料親合性的方法。細胞的黏附會影響細胞增生與分化。然而，量測細胞黏附力的設備大多需要精密的儀器，設備較複雜。所以我們設計一種簡單的動態施力設備，可快速測得細胞與基材的黏附力。利用已知力量的刮細胞量測技術來做細胞黏附力的比較。選用鈣磷生醫陶瓷與純鈦當作測試基材。因此本篇研究目的是設計量測細胞黏附力的方法，並以鈣磷生醫陶瓷與純鈦來觀察類骨母細胞的反應。實驗分成四部份：第一部份-確定射頻功率對鈣磷薄膜沉積性質的影響。以alpha-step、SEM、EDS和XRD分析結構與化學組成，實驗結果顯示披覆在試片上的鈣磷薄膜為非結晶相，經500℃熱處理後轉換為結晶相的鈣磷薄膜。第二部份製備兩種表面粗糙度，分別為以砂紙號數#180研磨與研磨到#1500號後使用Al2O3拋光。產生的表面粗糙度為(Ra = 0.48,0.04 micrometer)。第三部份-量測細胞在生醫陶瓷表面的黏附力，以我們設計的動態施力方式量測細胞黏附力並與刮細胞方式量測細胞黏附力作比較。實驗結果顯示細胞於Polish組黏附強度較高的為Polish CaP-h，次之為Polish和Polish CaP，與刮細胞的量測方式結果相同；#180組力量較高的為#180，次之為#180 CaP-h和#180 CaP與刮細胞方式相異，在統計上沒有顯著差異。由免疫螢光染色觀測細胞骨架經離心後無明顯變化。第四部份使用SEM評估細胞在不同條件試片上黏附型態，在Polish組和#180組可觀察到細胞於CaP-h表面黏附面積最大，其次是沒有鈣磷塗層的鈦，接著是CaP塗層試片。在Polish組細胞黏附面積與使用動態施力設備和刮細胞方式量測結果呈現正相關，然而較粗糙的#180組別在兩種方式的量測下無一致趨勢。因此推測動態施力設備可運用於較細緻的表面，不適合量測粗糙質較高的表面。此實驗結果得知CaP-h相對於CaP有較佳的生物活性而讓細胞展現較高的黏附力量。

Cell adhesion strength on biomaterials can assess biomaterials biocompatibility. Cellular attachment influences cellular proliferation and differentiation. However, quantitative methods for examining cell adhesion mostly need accurate instrument, which is relatively complicated. We develop a simple and easy technique to quantitatively measure the adhesion force between cells and substrate. To utilize cytodetachment technique to make comparison that cells adhesion strength. We select the calcium phosphorus bioceramics and commercially titanium as substrate to make comparison. The aim of this study was to develop a dynamic technique to quantitatively measure the adhesive force between cells and substrata. The study is divided into four parts. PartΙ. To determine the influence of discharge RF power level on the properties of the deposited Ca/P films on titanium substrates. The structure and chemical composition were investigated with alpha-step (thickness), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) and X-ray diffraction (XRD). The results showed that as-sputtered CaP coatings were amorphous, and 500°C heat-treated CaP coatings were crystalline. Part Ⅱ. Titanium alloy disks are prepared by wet grinding by grit silicon carbide paper #180 and wet grinding to #1500 then polishing by Al2O3 powder to the surface roughness with Ra values of 0.48 and 0.04 micrometer, respectively. Part Ⅲ. The measurement of cell adhesion force on bioceramics will be compared with dynamic applied forced and cytodetachment technique. The results showed that cell adhesion force on polish CaP-h more strength than polish CaP and Polish CaP that the same the cytodetachment. However, the cell adhesion force on #180 more strength than #180 CaP-h and #180 CaP that different to cytodetachment. Part Ⅳ. To evaluate the cells morphology in different conditions. Cells displayed well spreading on CaP-h coating than non-coated titanium and CaP. The cell attachment area and cell adhesion force is being connected on Polish group. However rougher #180 group not consistent tendency. Therefore, the dynamic technique utilize to smooth surface not roughness surface. This work shows that CaP-h film fave bettter bioactivity for cell adhesion.

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