點帶石斑魚(Epinephelus coioides)為台灣重要的經濟魚種之一，近年來，由於環境污染及氣候的變遷，加上神經壞死病毒(Nervous necrosis virus)和鰻弧菌(Vibrio anguillarum) 的感染，使得石斑魚因為逆境而造成死亡率居高不下，造成漁民重大的損失。根據研究指出，熱休克蛋白70家族為熱休克蛋白家族的成員之一，在細胞中的角色主要為伴隨蛋白(Chaperone)，會在逆境刺激時會大量的表現，其功能為協助細胞中的蛋白質作摺疊、移動位置或是避免蛋白質的錯誤折疊發生。點帶石斑魚的熱休克蛋白70家族中的HSC70以及HSP70基因之cDNA已選殖成功，在熱休克反應刺激下，HSC70和HSP70表現量都有上升的情形，另外利用鰻弧菌及低溫逆境刺激時，HSC70的表現量則沒有太大的改變，但是HSP70則會有被誘導的情形。目前已成功的利用細菌進行HSC70的蛋白質表現，並生產出能作用的anti-HSC70的抗體。在健康的石斑魚之中，HSC70 在頭腎、脾臟、肝臟中的表現量最高。另外也觀察到HSC70會表現在石斑魚鰭細胞的細胞質的部分。
關鍵詞：石斑魚、逆境、神經壞死病毒、鰻弧菌、HSC70、HSP70、熱休克

Orange-spotted grouper (Epinephelus coioides) is one of the important economical cultivate fish in Taiwan. In recent years, environment pollution, climate change and Nervous necrosis virus (NNV) or Vibrio anguillarum infection causes massive mortality in grouper juveniles and results in large quantity of damage to fisherman. According to previous study, Heat shock protein 70 family is a chaperone to refold protein, translocation and assist the correct folding of nascent and stress-accumulated misfolded proteins, and preventing their aggregation in the cell. In the previous work, grouper of HSC70 and HSP70 complementary DNA had been cloned. Under heat shock response, we find out that the expression of HSC70 and HSP70 increased, however, the expression of HSC70 have no markedly changes when Vibrio anguillarum and cold shock stimulate grouper, but HSP70 are induced by Vibrio anguillarum and cold shock stimulation. Now, we have finished HSC70 protein expression by the E. coli BL21(DE3) system, and produced the anti-HSC70 antibody successfully. In healthy groupers, the expression of HSC70 is abundant in head kidney, spleen, and liver. Also, we can speculate that HSC70 express in cytosol of grouper fin cells.
Key words: grouper, nervous necrosis virus, HSC70, HSP70, heat shock, Vibrio anguillarum

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