根據國際糖尿病聯盟(IDF)報導，2019年全球已有4.63億人患有糖尿病。糖尿病患者會出現如蛋白尿、視力衰退及手腳麻痺之併發症。此外，有時更嚴重危及生命安全之症狀，如失明及截肢，其中傷口癒合緩慢為糖尿病患者最常出現之問題。然而，過去由於研究豬隻傷口癒合太快速，不易看出藥物效果之差別。故本研究應用H-04及BE酵素在癒合較慢的糖尿病動物上。首先以alloxan建立高血糖的糖尿病蘭嶼豬模式及傷口開創。於結果顯示，在誘導一個月後，血糖值高於400 mg/dl且血糖沒有下降的糖尿病豬有六頭。而且，誘導後的糖尿病豬也發現體重減輕、脂肪減少，且胰臟組織也發現大量的胰島細胞死亡。而在正常豬與糖尿病豬傷口癒合方面，糖尿病豬在2.5 × 2.5 cm2傷口癒合方面有緩慢之趨勢，且傷口血流訊號值偏低、膠原蛋白沉積緩慢。接下來在第0天即放入含有0 - 500 IU/mL濃度之H-04的DW matrix於糖尿病豬的6 × 6 cm2之傷口，並在開創傷口後3 - 9天再利用BE酵素對傷口進行去腐皮。整體結果顯示，含有50 IU/mL之H-04的DW matrix且搭配BE酵素治療相比於其他劑量有傷口閉合及收縮較快之趨勢。綜合來說，含有H-04 50 IU/mL的DW matrix加速傷口癒合且搭配BE酵素可以在傷口癒合早期階段加速去除腐皮。

According to the International Diabetes Federation (IDF) report, there were 463 million people worldwide in the face of diabetes in 2019. Once patients due with diabetes, they have to experience complications such as proteinuria, vision loss, hand, and foot paralysis. Moreover, it sometimes happens with more serious life-threatening symptoms like blindness and amputation in which slow wound healing is the most common problem in diabetic patients. However, in the past, due to the wound healing too fast on the pig wound, it’s difficult to distinguish the drug effect. Thus, in this study, the H-04 and BE enzyme was used to apply on the diabetic animals with slower healing. Alloxan first was used to establish a model of the diabetic Lanyu pig with high blood sugar with the wound. The results showed there were six diabetic pigs which have blood glucose higher than 400 mg/dl and there was no decrease in blood glucose level after one month of induction. Also, induced-diabetic pigs have been found in weight loss, adipose tissue reduction, and a huge number of cell dead in pancreas. From the point of view of wound healing between the diabetic pig and the normal pig, the diabetic pig tended to heal slowly at 2.5 × 2.5 cm2 wounds, with low blood flow signal values and slow collagen deposition. Next, Diabetes Wound (DW) matrix consists 0 - 500 IU/mL of H-04 was used to put on the wounds at the sizes of 6 × 6 cm2 in the diabetic pig at day 0 and BE enzyme was used to peel skin from day 3 to day 9 post-operative. The results showed among of them, H-04 50 IU/mL in DW matrix and BE enzyme treatment showed a faster tendency of wound closure and wound contraction than other groups. In summary, DW matrix consists H-04 50 IU/mL accelerates the effect of wound healing and BE enzyme promotes in peeling dead skin in the early stage of wound healing.

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