本研究乃利用自製之內視鏡用無針式注射系統，以HPMC(羥丙基甲基纖維素)、MC(甲基纖維素)、甘油、褐藻膠為工作流體進行生物體之組織抬升，實驗乃是以內視鏡用無針式注射器緊貼待抬升之組織表面進行生物體組織噴注及抬升之作用，並利用數位相機記錄組織抬升及其消散之過程，以探討不同的工作流體進行組織抬升後液體消散之情形及無針式之噴注及注射特性。使用之生物體組織為足齡約六個月大之豬胃，主要之參數包含噴注量、速度、功率、注射位置、組織抬升高度、抬升消散時間等。為使組織成功抬升，第一步驟為穿透胃黏膜層，第二步驟為將抬升溶液順利注入，使用1650mm長的內視鏡管做體外動物實驗，在不同噴注壓力、不同噴注時間下成功穿透豬胃黏膜層的條件為噴注壓力最小為80bar，相當於最小噴注功率2.8w。另外，比較內視鏡工作流體輸送管長度1650mm與2350mm之兩種應用實例，結果顯示2350mm管長之實例所需噴注功率遠低於1650mm之實例，故之工作流體在系統中的能量損失，主要消耗於輸送管的部分。組織抬升之第二步驟需將抬升溶液注入次黏膜層，實驗結果發現，羥丙基甲基纖維素黏滯性越高時，抬升時間越長，當羥丙基甲基纖維素濃度大於0.5%(wt%)時，初始高度為10.6mm，其半衰期大於60分鐘，此時間足夠醫護人員操作手術。使用相同黏滯係數(20cP)的不同溶液，包括羥丙基甲基纖維素、褐藻膠、甘油等做為抬升溶液，初始抬升高度無顯著差異，但羥丙基甲基纖維素的半衰期為最長(超過60分鐘)。將抬升後的豬胃組織浸於福馬林使其硬化固定，再以H&E染色，證實無針式噴注之位置深達生物體組織之次黏膜層，故可以將組織之黏膜層抬升，使病灶與正常組織有效分離，以利後續內視鏡醫療手術之順利進行。

This paper investigates the characteristics of endoscopic needle-free injection and the performance of tissue elevation by an endoscopic needle-free injection system. We choose the stomach of six-months-old pig as the test material, with HPMC, MC, glycerin and alginate as the working fluid. The parameters include the injection mass, average power of injection, and the height of tissue elevation, and the evolution of tissue elevation. Longer duration of tissue elevation of the submucosal layer is maintained under the pressure of 90bar and the concentration of HPMC at 0.5%. The tissue elevation is 10.6mm suitable for medical surgery with the higher injection pressure under the same concentration of the working fluid because of the increased injection power. Analysis on the evolution of tissue elevation found that duration time is more than 60 minute under higher concentration of HPMC. The duration of tissue elevation is longer for higher concentration of the working fluid because the higher viscosity under high concentration would result in the elongation of the elevation time. A clear separation of the mucosal layer from the muscle layer is achieved by injecting HPMC in the samples with formalin-fixed and paraffin-embedded sections stained with H&E. Results of the experiments would provide the data base for endoscopic surgery in order to assure a successful operation.

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