中風為現代人的十大死因之一，對於社會的各方面造成很大的影響。因此針對其機制和藥物治療的探討是刻不容緩的。現在利用一項整合型的技術－微流體晶片，其優點為所需樣本量少、分析時間快且具高效率的分離，所以有應用在細胞層級分析的潛力。使用細胞模式的實驗，好處在於可以在重複性的控制環境下，測量細胞在某特定外在刺激改變之下，生理途徑隨之反應形成的變化，造成傳導物質分泌的不同，得到其生理上的意義。此外，在檢測系統上，電化學偵測的高靈敏度、便宜且方便和其它系統整合，可利用它去偵測dopamine, catechol, histamine, nitric oxide等具有氧化還原性質之神經傳導物質。上述技術，欲將整個系統建立在一個微小的晶片上，做為一個藥物和細胞生理反應的實驗平台。但目前使用PDMS製成的毛細管微管道，其表面性質呈現疏水性，不容易使分析的溶液注入管道，分離的效能也比材質以玻片製成的晶片差，因此，有許多研究即在討論如何改善PDMS的性質。本篇是利用有機溶劑ethyl acetate和acetone萃洗PDMS，可將PDMS在聚合時產生的oligomers萃洗出來，再以氧電漿修飾管道表面，可有效改善微管道原本的疏水性，得到親水性的表面，比起以往單單只以氧電漿修飾的PDMS，維持較長時間的親水性。使用Current-Monitoring Method測量電滲透流的大小，得到電滲透流的大小是5.0 × 10-4 cm2/Vs，其效果可維持至少5天；此外，對於本晶片偵測條件的最佳化，採用緩衝液20 mM MES （pH 6.0）; 分離電場強度100 V/cm ; 偵測電位0.7 V; 注入時間25 s。在此條件下去偵測神經傳導物質dopamine，目前可偵測至0.1 M，訊號響應時間約在分離後75 sec可以得到。

Stroke is the major reason that influences the health of people in modern society. Therefore, it is very important to investigate the pathological mechanism and protective method. The purpose of this paper is to investigate an integrated technique, capillary electrophoresis microchip, which includes the advantages of separated samples quickly, high efficiency, small volume of sample and low cost. Now it is applied to the analysis of cell scale. To use experiments of the cell model, we can get the more regular result under control. Cells secrete different concentrations of neurotransmitters under the stimulus. The electrochemistry is a high sensitive and convenient tool for detecting the neurotransmitters which have oxidative or reductive property such as dopamine, catechol, histamine, nitric oxide. We want to integrate these techniques on a chip to be a plate form for medicinal test and physiological mechanism. PDMS is extracted in a series of solvents designed to remove unreacted oligomers from the bulk phase. Second, the
oligomer-free PDMS is oxidized in a simple air plasma to improve the hydrophobic property. The electroosmosis flow is 5.0 × 10-4 cm2/Vs and it can last 5 days at least. We try many conditions to find the optimization of the microchip and then detect the 0.1 M dopamine and the signal appears in 75 sec after the separation when the experimental conditions are 20 mM MES buffer (pH 6.0), 25s injection time, 100V/cm separation voltage field, and 0.7 V detection voltage (vs. pseudo Pt).

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