近年因為能源危機及全球暖化的問題，各國皆積極在尋找乾淨且可再生之替代能源，其中生質能更是成為積極發展的重點，主要是因為生質能為"碳中性"的能源，製造過程為碳循環的一部分，並不會造成額外的二氧化碳生成，其中生質酒精就是其中一種。然而目前科技利用纖維料原生產生質酒精時，大約只有75~80%的電子能轉移至生質酒精上，意指大約還有20~25%的能源在殘渣中尚未被利用，若能有效利用殘渣，不但能處理廢棄物問題，更能從中回收能源再利用。故本研究將針對利用蔗渣作為生質酒精原料後之廢液，將其作為基質進行生質氫氣與甲烷之發酵，此批廢水之總化學需氧量(Chemical Oxygen Demand ,COD)約30 g/L，含有大量的有機物質，其中碳水化物的部分佔30 %，有機酸佔33%及乙醇佔12%，十分適合利用利用兩階段式生質能源程序來處理。
在第一階段連續式產氫發酵槽中，共操作了9個試程，發現在體積負荷(volumetric loading rate, VLR)為180 kg COD/m3/day的操作條件下有最佳的產氫表現，比產氫速率(specific hydrogen production rate, S.HPR)為37.64 ml/H2/g VSS/hr，氫氣產率(hydrogen yield)則為29.21 ml H2/g COD，氫氣組成約有38%，產氫速率及氫氣產率可做為日後實場化的工程依據。在厭氧產氫的批次中，得知縮短水力停留時間(Hydraulic Retention Time, HRT)能有效提升氫氣產率及微生物對於基質的負荷量，另外基質的負荷及乙酸化(Acetogenesis)也是影響產氫的重要因子。在甲烷發酵槽方面，當體積負荷為3.7 kg COD/m3 /day時有最好的產氣速率表現，其甲烷生成速率(methane production rate)為1.12 L CH4/L/day。
在整體兩階段操作完後COD去除率可達87.1%的，其中電子轉移至氫氣及甲烷的比例分別為0.3%及78.9%，顯示此兩階段式生質能源程序，能有效進行生質能源的再利用。

Global warming and energy crisis have already been proved as internal issues in recent years. The governments paid much attention to finding a clean and renewable energy. Therefore, developing biomass energy is main purpose for many counties. Biomass is considered as a carbon neutral source of energy because the carbon dioxide released into atmosphere by using biomass is recovered again by growth of new biomass. Bioethanol is one of biomass energy. Current technologies can only utilize 75-80% of the energy during cellulosic bioethanol fermentation, which implies that 20-25% might be wasted as residues. Thus, this study has focused on the energy recovery from cellulosic bioethanol residues. The characteristics of the bagasse alcohol fermentation residue were studied. The COD was 30000 mg/L, with a large amount of organics. For the electron distribution, carbohydrates comprised 37% of the total COD, and this portion can be fermented to hydrogen. On the other hand, the remaining organic acids (12%) and alcohols (42%), together with the volatile fatty acids produced in hydrogen fermentation, can further be utilized by methanogens to produce methane as energy product. Therefore, a two-stage bioreactor with hydrogen fermentation and methanogenesis was established in this study to treat this bagasse alcohol fermentation residue.
There were nine runs in the CSTR anaerobic hydrogen fermentation tank. The maximum specific H2 production rate and yield were investigated to be 37.64 mL H2/g VSS/hr and 29.21 mL H2/g COD when volumetric loading rate (VLR) was 180 kg COD/m3/day. By carrying out batch experiment, shortening HRT could enhance hydrogen yield and the loading of microorganism to substrate .Acetogenesis and S0/X0, or F/M ratio in CSTR, were found to be important factors on the efficiency of biohydrogen production. B/A ratio has been used as an indicator for evaluating the effectiveness of biohydrogen production. The best performance of the methane bioreactor in the view of methane production was obtained under VLR of 3.7kg COD/m3/day, in which 1.12 L CH4/L/day was achieved.
Approximately 87.1% of the COD in the bioethanol-fermentation residues was removed, with about 0.3% and 78.9% of it were recovered in the forms of hydrogen and methane. Results indicate that cellulosic bioethanol residues are also suitable for hydrogen and methane fermentation.

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