台灣鯛(Taiwan Tilapia)是具有潛力的高經濟價值之魚種，其魚鱗經由萃取後，可以萃取出膠原蛋白，轉變為具有非常高經濟價值的產品，本研究探討膠原蛋白萃出率與膠原蛋白之生物活性。台灣鯛魚鱗經由100℃60分鐘高溫水煮後得到膠原蛋白水解液，利用Superdex peptide® 10/300 column進行分析，能得到的分子量平均分配在大於12500Da、12500Da ~6511Da、與6511Da~1355Da這三個區間，如果細看圖的話，可以得知主要都分布在4500Da以上。進行抗氧化測定，從濃度9mg/ml來看，清除自由基的能力、還原力、螯合銅離子能力，分別可以達到49.65%、0.67 (OD 700nm)、69.89%。將經過前處理(未經過高溫加熱)的台灣鯛魚鱗再以Bromelain、papain及Flavourzyme這三種酵素分別進行萃出率探討，得到Bromelain:魚鱗=0.01:1、papain:魚鱗=0.01:1、Flavourzyme:魚鱗=0.02:1(重量比)且在50℃下反應達7小時能得到最佳的條件。以Superdex peptide® 10/300 column進行分析，可以發現到三種酵素的分子量會集中於6511Da ~ 1355Da這個區間來，而且隨著水解時間的增加，小於1355Da的分子量區間會逐漸增加。而在抗氧化的測定上，清除DPPH的能力三種酵素水解後都有明顯的上升，其中Flavourzyme所得之水解液能上升到80%的清除率，而還原力方面則是三種酵素都低於0.5(OD 700nm)，甚至水解時間的增加，有略微往下降。螯合銅離子能力，則是在三種酵素水解後都有明顯的提升，幾乎都在80%以上，而隨著酵素水解時間的延長，並沒有明顯的再提升。最後將膠原蛋白水解液進行小白鼠創傷傷口的癒合情形實驗，發現膠原蛋白水解液雖然對於傷口的癒合情形沒有明顯的幫助，但是有塗抹膠原蛋白液的部分對於傷口周圍的毛髮生長有明顯的生長。

The fish scale was once thought of a kind of trash for Taiwan Tilapia, Right now, it has become a convient source of collagen. In this study, we focus on the extraction and hydrolysis of the scale collagen, then investigate the relationship between the bioactivity and the molecular distribution of hydrolyzed peptide. The cleaned scales were autoclaved for 60 min. The molecular weight distribution of the liquid portion was analyzed by using Superdex peptide® 10/300 column, and found that there were three distributions, 12500Da, 12500Da~6511D, and 6511Da~1355Da, centered at 6511Da~1355Da. The ability for scavenging free radical, reduction, and chelating with Cu2+ were 49.65%, 0.67(OD 700nm), and 69.89%, respectively. The autoclaved scales was incubated at 50℃ for 7 hours with the presence of Bromelain, papain, and Flavourzyme, respectively, it was found the major distribution of molecular weight of the hydrolyzed peptides was centered at 6511Da ~ 1355Da. And the bioactivity was found increased by about 60% for scavenging the free radical and 30% for reduction. With the increase of hydrolyzed time, the bioactivity increases, indicating that the peptides are shorter. The assay to test the curing effect of the cut wound of rat, it was found that there was no significant difference compared with the control one; however, it was observed that the hydrolyzed fish scale collagen has effect on stimulate the growth of hair around the cut wound.

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