民間傳統使用香椿(Toona sinensis Roem)來調降血糖與血脂。研究指出餵食香椿葉萃取物給予高血脂症小鼠，顯著降低小鼠血清總膽固醇、三酸甘油脂(TG)、低密度脂蛋白(LDL)、增加高密度脂蛋白(HDL)，且降低LDL/HDL的比值，然而其降血脂分子機制仍未明瞭。因此本研究主要探討香椿萃取物 (Toona sinensis leaf extract, TSLE)對高脂飲食 (High Fat Diet, HFD)小鼠胰島素敏感性、脂肪細胞分化及脂質代謝相關蛋白表現量的影響。使用TSLE分別處理分化初期與分化後成熟的3T3-L1脂肪細胞，，以 Western blotting檢測分化及脂質代謝相關蛋白的表現、Real-Time PCR檢測分化相關基因、脂質生成相關基因與脂肪酸分解、氧化代謝相關基因的表現、偵測葡萄糖消耗率、觀察脂質累積、檢測活化PPARα活性，探討TSLE對脂肪細胞脂質代謝的調控。同時以HPLC進行TSLE成分鑑定，利用luciferase assay篩選成分活化PPARα活性，將有活化PPARα功能的成分處理3T3-L1，探討各成分對脂質代謝的調控。結果顯示，TSLE經由調節脂肪細胞脂質分解及脂肪酸氧化代謝路徑降低脂質累積，可開發成降血脂或減少體脂肪累積功能之保健產品。

In the present study, HFD mice and 3T3-L1 adipocytes were used to investigate the mechanism of Toona sinensis leaf extrcts (TSLE) on lipid metabolism. High-perfomance liquid chromatography (HPLC) and PPARα-LBD luciferase assay were used to investigate the functional compounds of TSLE. Results showed that TSLE decreased lipid accumulation in adipocyte via the regulation of lipolysis and fatty acid oxidation, suggesting that TSLE is potential to develop into a function food for hypolipidemia and decreased body-fat accumulation.

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