台灣漁業養殖是相當重要的經濟的一環，尤其具高經濟水產生物的疾病與治療被受重視，在全球漁業的型態轉變下，養殖漁業成為主要水產來源。在追求更高產量與獲利下，養殖產生幾項問題，如:高密度養殖、進口貿易種苗與親緣過近的繁殖育種，產生許多水產疾病爆發，加上氣候異常等環境因素導致疾病越來越容易爆發，造成經濟嚴重損害。若能在養殖現場即時與定時地進行檢測監控，即能有效的防範預防與即時治療，在未來生物醫療趨勢，必然以防範預防、即時檢測和即時治療為目標並利用整合微流體生物晶片分析技術來實現。本研究介紹了整合重組酶聚合酶擴增及側流試紙(RPA-LFD)於快速微流體生物晶片來檢測蝦白點病。檢測方法主要是利用RPA機制進行核酸檢測，結合LFD的終點檢測，將兩個步驟整合於微流體生物晶片。能在15分鐘內完成檢測及判讀，並隨著感染的嚴重程度不同，後續可以透過軟體讀取試紙條的灰階數值來進行分析。整個系統不僅能用於現場即時檢測，也可應用於其他生物疾病檢測。藉由本研究的整合系統更進一步的實現監控與現場檢測的需要，讓漁民能時刻維護養殖生物的健康，達到降低死亡率並提生產率之目的。

Fish farming or pisciculture has important contribution in the economy of Taiwan. With the transformation of global fisheries, the main source of aquatic products comes from aquaculture sectors. In the pursuit of higher productivity and profits, aquaculture sectors have encountered with several problems. As a result, many aquatic disease outbreaks have occurred, and also the environmental factors, such as climate abnormalities have caused more diseases. These outbreaks are responsible for serious economic damage. If it can be detected periodically with real-time monitoring system in the breeding site, we can effectively prevent these outbreaks and also have the enough time for medical treatment. The future biomedical trend is bound for prevention, detection and immediate treatment for the immediate objectives using different advanced integrated microfluidic bio-chip analysis techniques. This study incorporates the integration of recombinase polymerase amplification and lateral flow dipstick (RPA-LFD) in a rapid microfluidic biochip to detect shrimp white spot syndrome virus. The different levels of the infection can be detected through the grayscale value of the customized strip and can be interpreted completely with a time less than 15 minutes by a software analysis tool.

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