過去的研究證明機械力的刺激對於組織之發展與成熟有著決定性的影響，為了能夠在組織工程血管養成的階段中給予機械力刺激，我們需要一個有彈性之管狀支架。另一方面，我們假設支架本身的結構對於所養成組織之結構息息相關；因此，我們參考天然血管之微結構設計此一支架的微結構。我們選用生物可降解性，且富彈性之聚己內酯，作為支架的材料。利用靜電紡絲法，配合旋轉滾筒紡絲收集方式，以製備具方向性之纖維薄膜，再將薄膜捲成中空管狀物作為血管支架，本研究整合了細胞片與支架的理念，設計模擬天然血管中層結構。我們使用光學顯微鏡及電子顯微鏡來檢驗各種條件下所產生之支架微結構，並利用客製的力學測試裝置量測該支架的力學性質。為了解該支架的生物相容性及纖維排列對於細胞的影響，我們將3T3纖維母細胞植在纖維薄膜的表面，再將此含細胞之薄膜捲成中空管狀物，在培養一週與兩週後，以組織切片來觀察細胞的生長形態。
結果顯示，當支架的纖維方向越偏向環形方向，其環向的彈性模數會比較高，而軸向受力會比較小。反之，當支架的纖維方向越偏向軸向，其軸向的受力會比較大，而其卻有比較好的順應性。從培養一週跟兩週的含細胞支架的切片中，我們發現細胞生長狀態良好，且會沿著纖維排列方向生長。

The results showed that elastic moduli of scaffolds in circumferential direction were closely related to the fibers orientation. Scaffolds with fibers aligned toward the circumferential direction showed higher elastic moduli than those aligned toward the axial direction. On the other hand, these scaffolds with fibers aligned toward the circumferential direction appeared less stiff in the axial direction in comparison with those with fibers aligned toward to the axial directions. The H&E staining of the constructs cultured for one or two weeks showed that cells appeared to grow well in the scaffold and, more importantly, they aligned to the prescribed fiber directions. This demonstrated that the scaffold has a potential to guide cell orientations and hence the tissue structure.

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