由實驗室前人的研究結果，已知海洋綠藻：Picochlorum sp. strain S1b (簡稱 S1b) 與 3 株海洋細菌：Labrenzia sp. strain #8、Muricauda sp. strain #50 及 Arenibacter sp. strain #61 共培養形成藻菌組合體後，能明顯抑制副溶血弧菌生長本研究的目的即在探討前述藻菌組合體抑制弧菌生長的機制。為確認其抗菌能力是否和抗菌物質的分泌有關，因此將前述的藻菌組合體進行混營培養，利用各種萃取搭配 well diffusion assay 確認活性位置的方法進行純化，利用質譜及核磁共振法確認結構，結果鑑定出一個含特殊2,6鍵結的雙葡萄糖和 adenosine 兩種成分，然而這些城焚可能是培養基的內容物或衍生物。為了避免培養基內有機質的干擾，後續實驗中改採無機的培養基來培養S1b的藻菌組合體，同時嘗試利用在組合體內添加弧菌的方法來誘發抑菌活性的產生，結果發現組合體雖然確實能明顯抑制弧菌，然而移除藻菌細胞的上清液卻沒有明顯的抑制弧菌效果，說明抑制活性來自藻菌細胞，而非培養基 (外泌物質)，嘗試破菌來測試藻菌細胞內活性，發現破菌後的上清液有明顯抑菌活性，發現抑菌物質可能存在於藻菌細胞內。

Algal-bacterial consortium is considered to be an effective control method to inhibit the growth of vibrio. Based on the results of previous studies in the laboratory, it is known that marine green algae: Picochlorum sp. strain S1b is combined with three marine bacteria: Labrenzia sp. After strain #8, Muricauda sp. strain #50 and Arenibacter sp. strain #61 are co-cultured to form an algal-bacterial consortium, it can significantly inhibit Vibrio parahaemolyticus (reduce the number of viable cells). The purpose of this study is to investigate the mechanism of vibrio parahaemolyticus growth inhibition by S1b algal-bacterial consortium. First, in order to confirm whether its antibacterial ability is related to the secretion of antibacterial substances, various extraction methods were used to purify the algal-bacterial consortium broth. use mass spectrometry and nuclear magnetic resonance. The structure was confirmed by the method, and the result identified a special 2-6 Disaccharide and adenosine. In order to avoid the interference of organic matter in the medium, in the subsequent experiments, inorganic Walne′s medium was used to cultivate the S1b algal-bacterial consortium. Analyze the antibacterial activity of the culture medium by the checkerboard assay at the same time, try to use the method of adding V. parahaemolyticus to the algal-bacterial consortium to induce antibacterial activity. It turns out that although the algal-bacterial consortium can obviously inhibit V. parahaemolyticus, the supernatant from removing the algae cells has no obvious inhibitory effect on vibrio. Try to use sonication to break the cell to test the intracellular activity of algae. It was found that the supernatant after breaking had obvious antibacterial activity, and it was found that the antibacterial substance may be present in algae cells.

丁楹驊，橙黃壺菌BL10之多元不飽和脂肪酸合成酶的分析和純化，國立成功大學生物科技與產業科學系碩士論文，2019。

郭榮哲，微藻 Picochlorum sp. S1b 抑制弧菌活性生長的研究，國立成功大學生物科技所碩士論文，2012。

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