本研究主要探討蛋白質打結構形與其訊息RNA(mRNA)模版構形之空間關連性。從蛋白質資料庫(PDB)中篩選出18個打結蛋白質，並針對這18個蛋白質打結的構形去推測其mRNA 模版構形。此外，轉譯過程中，參與蛋白質合成的關鍵因素有胺基酸、tRNA、mRNA、以及核糖體…等，我們利用Hyper Chem軟體排列這四個分子的相對應空間立體結構。在蛋白質打結的區域，排列出mRNA 環(loop)，並測量的結果顯示核糖體多為無法通過打結環的空間大小。在mRNA模版構形上，利用tRNA的苜蓿葉形結構，排列出不打結的mRNA模版構形就可能形成打結的蛋白質結構。因此，綜合上述結論，我們推論出蛋白質打結的構形，與其mRNA是否含有打結的構形可能是沒有直接的關連性。並且發現在打結蛋白質打結處的二級結構皆為摺板構形。

This study aims at investigating the conformational relationship between knotted proteins and corresponding mRNA templates. Particularly, we focus on resolutions to the following question: Is a knotted protein necessarily translated from a knotted mRNA? For this purpose, we first chose 18 knot proteins from PDB (protein data bank) to speculate the conformations of mRNA templates according to the knot conformation in 18 proteins. In addition, the key molecules in protein synthesis process are amino acids, transfer RNA, messenger RNA, and ribosome. The software Hyper Chem was used to figure out the structures of these four molecules. After the mRNA knot loop was made, we found the 3D space is too small to make the ribosome pass through in protein knot region. Taking more considering, a mRNA structure without knot conformation can be designed with the conformation of mRNA template and the cloverleaf structure of tRNA. Therefore, we speculated that there could be no direct relation between the knotted protein and its mRNA template conformation. More, the secondary structures of knot position are all beta sheet.

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