本研究成功開發鎳鈀合金微孔洞薄膜過濾晶片，並以此晶片將血球(Blood cells)與血漿(Plasma)分離。研究策略是利用掃流過濾(Cross flow filtration)來分離血液中的血球細胞，掃流過濾的原理是當流體平行流經過濾薄膜平面，而產生平行剪應力來掃除堆積於過濾薄膜表面的粒子，使得小於濾膜孔徑的分子隨著時間仍能維持高濾速穿過濾膜，而不增加操作阻力。本實驗利用微機電製程技術 (Micro electro mechanical systems, MEMS)製作出聚二甲基矽氧烷(Polydimethylsiloxane, PDMS)掃流區晶片及收集區晶片，而鎳鈀合金微孔洞薄膜則使用微電鑄技術(Micro-electroforming)製作，最後將鎳鈀合金微孔洞薄膜與PDMS晶片結合，即完成鎳鈀合金微孔洞薄膜過濾晶片。本研究將對不同全血稀釋倍數(10倍、5倍、3倍、2倍及未稀釋)、不同薄膜孔徑(1 µm、2 µm、4 µm、7 µm及10 µm)及不同流量(1 mL/min、3 mL/min、5 mL/min、7 mL/min及10 mL/min)去進行血球過濾率的探討。由實驗結果發現，當薄膜孔徑為2 µm且流量達到10 mL/min的情況下，則血球過濾率則可達最佳過濾率96.2%，所以鎳鈀合金微孔洞薄膜孔徑及流量大小對於血球過濾率有影響性。最後，本鎳鈀合金微孔洞薄膜過濾晶片以免疫球蛋白E(Immunoglobulin E, IgE)過敏患者血液作為臨床檢測分析目標，以酵素免疫分析方法(Enzyme-linked immunosorbent assay, ELISA)分別對傳統離心機與過濾晶片分離出的血漿進行呈色分析，結果顯示上述兩種血液分離方法所分離出的患者血漿具有相同的檢測趨勢。本研究所開發的過濾晶片有別於一般傳統離心機與過濾晶片，用於分離血球與血漿有著良好的過濾效果，可在短時間完成過濾，並具備不易阻塞、不易溶血(Hemolysis)、低成本及容易操作等優點。

This study successfully develops the nickel-palladium alloy micro-porous membrane filtration chip and utilizes the chip to separate blood cells and blood plasma. The filtration chip is based on the cross flow filtration, and the fluid flows parallel to the membrane, and it generates parallel shear stress to remove the clogging microparticles on the membrane. The Ni-Pd alloy micro-porous membrane filtration chip uses MEMS process and casting molding to manufacture polydimethylsiloxane (PDMS) cross flow chip and reservoir chip. Then, combined Ni-Pd alloy micro-porous membrane with PDMS chips, in addition, the membrane is manufactured by micro-electroforming process. This study was discussed the filtration rate under different blood dilution times(original, 2X, 3X, 5X and 10X), different membrane pore sizes(1 µm, 2 µm, 4 µm, 7 µm and 10 µm) and different flow rates(1 mL/min, 3 mL/min, 5 mL/min, 7 mL/min and 10 mL/min). When the flow rate is 10 mL/min and pore size is 2 µm, the filtration rate is up to 96.2%. Finally, this study is focused on IgE allergy blood sample separation, and is compared centrifuge with filtration chip by ELISA, and the results of filtration chip have the same trend with centrifuge. This study is different to other filtration chip that it can rapidly finish separation, and avoid clogging and hemolysis, and has great effect on blood cells and plasma separation from the experimental results.

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