術後加速傷口癒合且減小疤痕大小一直是學者積極努力的目標。在過去，已有多種物理性刺激因子與敷傷材料被研究其對傷口癒合的影響；然而，鮮少研究以皮膚的力學性質作為傷口癒合指標。多種生物性材料已作為臨床上的使用，本研究使用大鼠傷口模型，將膠原蛋白(Collagen)、明膠(Gelatin)與透明質酸(HA)與其複合物等不同生物性材料注射於縫合的傷口，在1、2、4、6與8週分別以拉力測試測量皮膚拉伸強度作為皮膚癒合指標、使用組織切片染色了解癒合組織生長情況以及膠原蛋白定量觀察胞外基質的生成情況。
結果發現材料注射會有不同的疤痕外觀。癒合初期傷口在在單一材料注射會有縫線疤痕；複合材料則是有較大且向內微凹的傷疤。癒合晚期則複合材料有較相似於正常皮膚之外觀。力學性質恢復得知，HA較所有材料注射組有較高的力學性質恢復情況，其他組彼此間均無其差異性。組織切片觀察顯示，單一材料有較快的表皮恢復情況且真皮層組織內部結構較為緻密；複合材料則是具有較於相似正常皮膚真皮組織細部的構型。於組織內膠原蛋白生成量比例結果發現，除單一材料於癒合時期2週Gelatin與HA組有較高的分泌量之外，彼此間無明顯差異。
綜合以上結論發現，單一材料對於癒合初期表皮癒合與再上皮化程度提供顯著效果；癒合初期具有較佳的傷口密合，癒合晚期擁有較佳的皮膚拉伸強度與恢復情況，但存在隱約縫線的縫線疤痕。複合材料作用效果可能著重於癒合後期真皮結構的加速新生與重組。

Quick and smooth skin healing is the goal in wound healing researches. In the past, several physical stimulating factors and wound dressing materials have been studied for their influences on wound healing. However, only few studies investigated the mechanical recovery of healing wound. Several biological materials have been used in clinical practice. Natural materials such as Type I collagen, Hyaluranon (HA), gelatin and their composite are similar to the composition of natural skin and have beneficial potential in cell proliferation, differentiation and ECM synthesis at wound sites. So far, no research has reported the mechanical recovery of the healing skin by injecting those natural biomaterials directly into the wound site. The aim of this study was to investigate the mechanical recovery influences of injectable biomaterials such as collagen, HA, gelatin and their composite on incisional wound injury in rat model. Tensile strength of healing skin was measured at 1, 2, 4, 6 and 8 weeks after incision; than, the correlated recovery index was calculated. The architecture of skin-wound was observed with histological section and collagen synthesis was measured.
The results showed that different biomaterial injection provided different scar pattern. Mono biomaterial groups had suture-scar pattern and mixed biomaterial groups had sunken scar pattern. The HA group had the best recovery results of mechanical properties compared with other groups. Histological observations exhibited that mono biomaterial groups had faster epidermis recovery and more compact dermis architecture. The mixed group had similar dermis architecture to normal skin. Collagen synthesis ratio showed that Gelatin and HA group had a higher collagen content at 2 weeks of healing time. Overall, mono biomaterial had significant effects on epithelial wound healing and re-epithelialization during early stage. In the late stage, the skin had a better tensile strength and recovery index, but there were slight suture-scar at wound site. Effect of mixed biomaterials might focus on regeneration and remodeling of the architecture of skin-wound.

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