幾世紀以來在亞洲國家裡許多經證實具有對氣喘具有療效的傳統中草藥，包括單方及複方等，都是經以口服的方式來進行治療。在本實驗以卵蛋白素(ovalbumin)作為過敏原刺激已致敏化的天竺鼠，再施予氣霧化複方中草藥(CTCM)。我們所使用的複方中草藥命名為OTM66，此配方在經過煎煮後以霧化的方式讓實驗動物吸入。實驗結果顯示OTM66可以有效的抑制由OVA引發致敏化天竺鼠的氣喘反應包括呼吸道阻力、介白素-5 (IL-5)的濃度以及發炎細胞數量等的增加，並且發現OTM66也具有支氣管擴張劑的效用，另外β-腎上腺素受體的拮抗劑propranolol會減弱由OTM66對於已致敏化呼吸道阻力的抑制作用。此外本實驗還利用肥大細胞株P815作體外細胞實驗，以compound 48/80刺激P815引發細胞去顆粒化 (degranulation) 並釋放出組織胺以及介白素-4 (IL-4)等物質，經實驗結果發現經OTM66可以抑制P815所釋放的組織胺和介白素-4，透過顯微鏡觀察發現產生去顆粒化的P815也有明顯的減少。由此證實OTM66可以有效抑制氣喘的反應，包括立即性的支氣管收縮以及延遲性的發炎細胞增生等，而且此複方中藥之作用機制包括可以透過刺激β-交感神經受器來擴張支氣管，也可以透過穩定肥大細胞避免其去顆粒化的現象來抑制氣喘反應。

Since traditional Chinese medicines can effectively relieve symptoms of asthma, the agents have been used as oral medications for anti-asthma in Asian countries within centuries. In this study, we showed a novel medication by developing of a complex traditional Chinese medicine (CTCM) in nebulized form, so called as OTM66. OTM66 was applied through the intratracheal route and found to be effective against an early-phase air-flow obstruction in ovalbumin (OVA)-sensitized guinea pigs. The application of OTM66 inhibited IL-5 production in OVA-sensitized guinea pigs. We further examined the mechanism of OTM66 to determine its function in the early-phase response to OVA challenge. Using we found that the inhibition of bronchoconstriction by OTM66 was attenuated by pretreatment with the β-adrenergic antagonist propranolol, suggesting that OTM66 exerts a bronchodilator effect via the β-adrenergic receptor-dependent pathway. Moreover, OTM66 was able to inhibit histamine and IL-4 secretion in the OVA-induced airway hypersensitivity in guinea pigs at 5 min post-OVA challenge. The in vitro study using P815 revealed that treatment with OTM66 stabilized mast cells. In conclusion, our results indicate that OTM66 is a promising bronchodilator and works well as a mast cell stabilizer. The findings provide important evidence showing its application for the treatment of allergenic airway disorders.

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