結合傳統農業與現代科技的農企業 (agribusiness)被認為是全球最具前景的產業。其中蘭花是台灣農企業中最有價值的產品，於2015年蝴蝶蘭出口額高達1.76億美元。台灣為世界最大的蝴蝶蘭出口國，然而，蘭花於生長階段所需時間非常長，加上病原菌的入侵，容易造成很大的經濟損失。據前人研究表示，印度梨形孢真菌(Piriformospora indica)是一種類似叢枝菌根菌的內生真菌，最初發跡是從印度塔爾沙漠被分離出來，目前已知約可與150種植物做互利共生，並且促進宿主植物生長。除此之外，此真菌可以被培養於人工培養基，為了瞭解此真菌與蘭花共生的益處，首先我們使用三種蘭花：蝴蝶蘭 (Phalaenopsis aphrodite subsp. formosana)、扇形文心蘭(Erycina pusilla)、鐵皮石斛 (Dendrobium officinale)，分別在各式不同培養基中與印度梨形孢真菌共培養，企圖找出最合適的培養基。其次，於溫室30℃中將蝴蝶蘭接種印度梨形孢真菌，觀察其生長。最後，使用與印度梨形孢真菌共培養之蝴蝶蘭，測其對病原體軟腐病 (Soft rot)與褐斑病 (Brown spot)的抗性。原本預期與印度梨形孢真菌共培養後會促進蝴蝶蘭生長，以及提高對軟腐病及褐斑病的耐受性，然而，本試驗數據顯示，利用T1去糖、G10等培養基共培養印度梨形孢真菌與蝴蝶蘭、扇形文心蘭、鐵皮石斛，皆無法使印度梨形孢真菌對蘭花有促進生長現象。而在30℃環境下，接種印度梨形孢真菌對於蝴蝶蘭並沒有促生長的效果，此外，亦無顯著提升對於軟腐病與褐斑病之耐受性。對此，有別以往文獻結果，印度梨形孢真菌對於促進宿主植物的生長與增加宿主植物對病原菌的耐受性，根據我們的實驗，在30℃環境下，這並不作用於蝴蝶蘭、扇形文心蘭及鐵皮石斛。

Orchid industry, in particular, is one of the most valuable agricultural industries in Taiwan. Based on current research studies, Piriformospora indica, an arbuscular mycorrhiza fungus-like, and endophytic fungus, is reported to be capable of establishing symbiosis with plants and its interactions with plants promotes growth in approximately 150 plants. This study aims to elucidate the beneficiary roles of P. indica in symbiosis with orchids. Firstly, three different species of orchids, Phalaenopsis aphrodite, Erycina pusilla and Dendrobium officinale, were co-cultured with P. indica in different orchid growth media to determine the optimal media most suitable for symbiosis. Secondly, P. aphrodite grown in the greenhouse was inoculated with P. indica to evaluate its effect in enhancing orchid growth. Thirdly, P. aphrodite co-cultured with P. indica was infected with bacterial pathogens, Pcc and Aac to evaluate whether P. indica confers plant resistance against soft-rot and brown spot diseases, respectively. Despite the use of different orchid culture media, improvement in plant growth was not observed for the three orchids co-cultured with P. indica. In addition, the presence of P. indica neither enhances plant growth nor confers plant tolerance against pathogen invasion in the greenhouse. Our results demonstrate that the beneficial role of P. indica reported for many plants is not applicable to orchid varieties.

文紀鑾、郭昭麟，台灣藥用石斛研究與產業發展，農業世界雜誌370期，52-58，2014。

位國慶，蝴蝶蘭軟腐病之發生及其藥劑防治，台灣糖業研究所研究彙報133期，1-10，1991。

位國慶、陳文輝，溫度與相對溼度對軟腐病發生之影響，台灣糖業研究所研究彙報159期，43-57，1998。

林崇熙，小小蘭花真有趣，中央研究院週報1322期，6-8，2011。

邱翊恬、張正，扇形文心蘭生物學研究概況，植物種苗13期，21-30，2011。

金輝、許忠祥、陳金花、韓素芬、葛頌、羅毅波，鐵皮石斛組培苗與菌根真菌共培養過程中的相互作用，植物生態學報33期，233-239，2009。

許秀惠，臺灣花卉之細菌病害，中華植物保護學會特刊新二號，中華植物保護學會編印，63-75，1994。

黃德昌、李惠玲，蝴蝶蘭病害之發生與防治研究，台灣花卉病蟲害研討會專刊，中華植保學會編印，149-157，1994。

曹星星，鐵皮石斛病原真菌分離與鑑定及印度梨形孢促生作用研究，中國浙江大學碩士學位論文，1-80，2015。

陳玉蓮，台南市政府農業局，臺灣國際蘭展為世界蘭花交易重要平臺，2016。

陳俊位、鄧雅靜、蔡宜峯，木黴菌在作物病害防治的開發與應用，臺中區農業改良場特刊121期，87-115，2014。

陳俊位，木黴菌在農業上的應用，臺中區農業改良場九十九年專題討論專集， 67-72，2010。

張元聰、陳燿煌、韓錦絲，蝴蝶蘭設施栽培，台南區農業專訊67期，28-33，2009。

楊玉婷，全球蘭花發展現況與未來展望，農業生技產業季刊3期，36-41，2010。

謝廷芳，蘭花病害的介紹及防治，農委會農業試驗所，2007。

羅淑芳，台灣三種藥用石斛基原鑑定、大量繁殖及抗氧化作用之研究，國立中興大學博士論文，1-25，2004。

藍志帆，蝴蝶蘭抗細菌性軟腐病基因轉殖之研究，臺灣大學園藝學研究所學位論文，1 - 69，2007。

Al-Absi, K. & Al-Ameiri, N. Physiological responses of tomato to inoculation with Piriformospora indica under osmotic stress and chloride toxicity. International Journal of Agriculture and Forestry 5, 226-239, (2015).

Al-Absi, K. & Al-Ameiri, N. Physiological responses of tomato to inoculation with Piriformospora indica under osmotic stress and chloride toxicity. International Journal of Agriculture and Forestry 5, 226-239, (2015).

An, F.-M., Hsiao, S.-R. & Chan, M.-T. Sequencing-based approaches reveal low ambient temperature-responsive and tissue-specific microRNAs in phalaenopsis orchid. PLoS One 6, e18937, (2011).

Anith, K. N., Sreekumar, A. & Sreekumar, J. The growth of tomato seedlings inoculated with co-cultivated Piriformospora indica and Bacillus pumilus. Symbiosis 65, 9-16, (2015).

Baishya, D., Deka, P. & Kalita, M. C. In vitro co-cultivation of Piriformospora indica filtrate for improve biomass productivity in Artemisia annua(L.). Symbiosis 66, 37-46, (2015).

Bajaj, R., Agarwal, A., Rajpal, K., Asthana, S., Kumar, R., Prasad, R., Kharkwal, A. C., Sherameti, I., Oelmüller, R. & Varma, A. Co-cultivation of Curcuma longa with Piriformospora indica enhances the yield and active ingredients. American Journal of Current Microbiology 2, 6-17, (2014).

Baker, M. L. & Baker, C. O. Orchid species culture: Oncidium/Odontoglossum alliance. Timber Press Oregon, 992, (2006).
Bechtel, H., Cribb, P. & Launert, E. The manual of cultivated orchid species 3rd edn. Blandford Press, 1-7, (1981).

Carlsward, B. S., Stern, W. L., Judd, W. S. & Lucansky, T. W. Comparative leaf anatomy and systematics in Dendrobium, sections Aporum and Rhizobium (Orchidaceae). International Journal of Plant Sciences 158, 332-342, (1997).

Christenson, E. A. Phalaenopsis: a monograph. Portland, Oregon: Timber Press, 396, (2001).

Das, A., Kamal, S., Shakil, N. A., Sherameti, I., Oelmuller, R., Dua, M., Tuteja, N., Johri, A. K. & Varma, A. The root endophyte fungus Piriformospora indica leads to early flowering, higher biomass and altered secondary metabolites of the medicinal plant, Coleus forskohlii. Plant Signaling & Behavior 7, 103-112, (2012).

Das, A., Prasad, R., Srivastava, R., Deshmukh, S., Rai, M. & Varma, A. Cocultivation of Piriformospora indica with medicinal plants: case studies. Piriformospora indica, Springer Berlin Heidelberg, 149-171 (2013).

Dijk, E. & Eck, N. D. Effects of mycorrhizal fungi on in vitro nitrogen response of some Dutch indigenous orchid species. Canadian Journal of Botany 73, 1203-1211, (1995).

Dolatabadi Kari, H., Mohammadi Goltapeh, E., Mohammadi, N., Rabiey, M., Rohani, N. & Varma, A. Biocontrol potential of root endophytic fungi and Trichoderma species against Fusarium wilt of lentil under in vitro and greenhouse conditions. Journal of Agricultural Science and Technology 14, 407-420, (2011).

Fakhro, A., Andrade-Linares, D. R., von Bargen, S., Bandte, M., Buttner, C., Grosch, R., Schwarz, D. & Franken, P. Impact of Piriformospora indica on tomato growth and on interaction with fungal and viral pathogens. Mycorrhiza 20, 191-200, (2010).

Ghabooli, M., Khatabi, B., Ahmadi, F. S., Sepehri, M., Mirzaei, M., Amirkhani, A., Jorrin-Novo, J. V. & Salekdeh, G. H. Proteomics study reveals the molecular mechanisms underlying water stress tolerance induced by Piriformospora indica in barley. Journal of Proteomics 94, 289-301, (2013).

Ghanem, G., Ewald, A., Zerche, S. & Hennig, F. Effect of root colonization with Piriformospora indica and phosphate availability on the growth and reproductive biology of a Cyclamen persicum cultivar. Scientia Horticulturae 172, 233-241, (2014).

Gill, S. S., Gill, R., Trivedi, D. K., Anjum, N. A., Sharma, K. K., Ansari, M. W., Ansari, A. A., Johri, A. K., Prasad, R., Pereira, E., Varma, A. & Tuteja, N. Piriformospora indica: Potential and Significance in Plant Stress Tolerance. Frontiers in Microbiology 7, 332, (2016).

Harrach, B. D., Baltruschat, H., Barna, B., Fodor, J. & Kogel, K. H. The mutualistic fungus Piriformospora indica protects barley roots from a loss of antioxidant capacity caused by the necrotrophic pathogen Fusarium culmorum. Molecular Plant-Microbe Interactions 26, 599-605, (2013).

Jacobs, S., Zechmann, B., Molitor, A., Trujillo, M., Petutschnig, E., Lipka, V., Kogel, K. H. & Schafer, P. Broad-spectrum suppression of innate immunity is required for colonization of Arabidopsis roots by the fungus Piriformospora indica. Plant Physiology 156, 726-740, (2011).

Jogawat, A., Saha, S., Bakshi, M., Dayaman, V., Kumar, M., Dua, M., Varma, A., Oelmüller, R., Tuteja, N. & Johri, A. K. Piriformospora indica rescues growth diminution of rice seedlings during high salt stress. Plant Signaling & Behavior 8, e26891, (2014).

Johnson, J. M., Sherameti, I., Nongbri, P. L. & Oelmüller, R. Standardized conditions to study beneficial and nonbeneficial traits in the Piriformospora indica/Arabidopsis thaliana interaction. Piriformospora indica, Springer Berlin Heidelberg, 325-343 (2013).

Knecht, K., Seyffarth, M., Desel, C., Thurau, T., Sherameti, I., Lou, B., Oelmüller, R. & Cai, D. Expression of BvGLP-1 encoding a germin-like protein from sugar beet in Arabidopsis thaliana leads to resistance against phytopathogenic fungi. Molecular Plant-Microbe Interactions 23, 446-457, (2010).

Lahrmann, U., Strehmel, N., Langen, G., Frerigmann, H., Leson, L., Ding, Y., Scheel, D., Herklotz, S., Hilbert, M. & Zuccaro, A. Mutualistic root endophytism is not associated with the reduction of saprotrophic traits and requires a noncompromised plant innate immunity. New Phytologist 207, 841-857, (2015).
Lee, Y.-C., Johnson, J. M., Chien, C.-T., Sun, C., Cai, D., Lou, B., Oelmüller, R. & Yeh, K.-W. Growth promotion of Chinese cabbage and Arabidopsis by Piriformospora indica is not stimulated by mycelium-synthesized auxin. Molecular Plant-Microbe Interactions 24, 421-431, (2011).

Murphy, B. R., Doohan, F. M. & Hodkinson, T. R. Yield increase induced by the fungal root endophyte Piriformospora indica in barley grown at low temperature is nutrient limited. Symbiosis 62, 29-39, (2014).

Qiang, X., Zechmann, B., Reitz, M. U., Kogel, K. H. & Schafer, P. The mutualistic fungus Piriformospora indica colonizes Arabidopsis roots by inducing an endoplasmic reticulum stress-triggered caspase-dependent cell death. Plant Cell 24, 794-809, (2012).

Rabiey, M., Ullah, I. & Shaw, M. The endophytic fungus Piriformospora indica protects wheat from fusarium crown rot disease in simulated UK autumn conditions. Plant Pathology 64, 1029-1040, (2015).

Rasmussen, H. N. Recent developments in the study of orchid mycorrhiza. Plant and Soil 244, 149-163, (2002).

Satheesan, J., Narayanan, A. K. & Sakunthala, M. Induction of root colonization by Piriformospora indica leads to enhanced asiaticoside production in Centella asiatica. Mycorrhiza 22, 195-202, (2012).

Sharma, P., Kharkwal, A. C., Abdin, M. Z. & Varma, A. Piriformospora indica improves micropropagation, growth and phytochemical content of Aloe vera L. plants. Symbiosis 64, 11-23, (2014).

Sheehan, T. & Sheehan, M. Orchid genera illustrated: 127. Psygmorchis. American Orchid Society 58, 24-25, (1989).

Smith, S. E. & Read, D. J. Mycorrhizal symbiosis. Academic press, 800, (2010).

Sun, C., Johnson, J. M., Cai, D., Sherameti, I., Oelmuller, R. & Lou, B. Piriformospora indica confers drought tolerance in Chinese cabbage leaves by stimulating antioxidant enzymes, the expression of drought-related genes and the plastid-localized CAS protein. Plant Physiology 167, 1009-1017, (2010).
Sun, C., Shao, Y., Vahabi, K., Lu, J., Bhattacharya, S., Dong, S., Yeh, K.-W., Sherameti, I., Lou, B. & Baldwin, I. T. The beneficial fungus Piriformospora indica protects Arabidopsis from Verticillium dahliae infection by downregulation plant defense responses. BMC Plant Biology 14, 268, (2014).

Sweet, H. R. The genus Phalaenopsis. The Orchid Digest Orchids of the World 1, 128, (1980).

Unnikumar, K. R., Sree, K. S. & Varma, A. Piriformospora indica: a versatile root endophytic symbiont. Symbiosis 60, 107-113, (2013).

Varma, A., Bakshi, M., Lou, B., Hartmann, A. & Oelmueller, R. Piriformospora indica: A Novel Plant Growth-Promoting Mycorrhizal Fungus. Agricultural Research 1, 117-131, (2012).

Varma, A., Sherameti, I., Tripathi, S., Prasad, R., Das, A., Sharma, M., Bakshi, M., Johnson, J., Bhardwaj, S. & Arora, M. The Symbiotic Fungus Piriformospora indica. Fungal associations, Springer Berlin Heidelberg, 231-254 (2012).

Varma, A., Verma, S., Sahay, N., Bütehorn, B. & Franken, P. Piriformospora indica, a cultivable plant-growth-promoting root endophyte. Applied and Environmental Microbiology 65, 2741-2744, (1999).

Verma, S., Varma, A., Rexer, K.-H., Hassel, A., Kost, G., Sarbhoy, A., Bisen, P., Bütehorn, B. & Franken, P. Piriformospora indica, gen. et sp. nov., a new root-colonizing fungus. Mycologia, 896-903, (1998).

Waller, F., Achatz, B., Baltruschat, H., Fodor, J., Becker, K., Fischer, M., Heier, T., Hückelhoven, R., Neumann, C. & von Wettstein, D. The endophytic fungus Piriformospora indica reprograms barley to salt-stress tolerance, disease resistance, and higher yield. Proceedings of the National Academy of Sciences of the United States of America 102, 13386-13391, (2005).

Wu, P.-H., Huang, D.-D. & Chang, D. C. Mycorrhizal symbiosis enhances Phalaenopsis orchid’s growth and resistance to Erwinia chrysanthemi. African Journal of Biotechnology 10, 10095-10100, (2011).

Ye, W., Shen, C. H., Lin, Y., Chen, P. J., Xu, X., Oelmuller, R., Yeh, K. W. & Lai, Z. Growth promotion-related miRNAs in Oncidium orchid roots colonized by the endophytic fungus Piriformospora indica. PLoS One 9, e84920, (2014).