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研究生: 劉虹吟
Liou, Hung-Yin
論文名稱: 以電噴灑方式製造藥物微膠囊
Drug Micro-encapsulation Using Electrospray Technique in a Planar Polymer Microchip
指導教授: 楊瑞珍
Yang, Ruey-Jen
學位類別: 碩士
Master
系所名稱: 工學院 - 工程科學系
Department of Engineering Science
論文出版年: 2010
畢業學年度: 98
語文別: 中文
論文頁數: 70
中文關鍵詞: 電噴灑錐狀噴流模態微膠囊
外文關鍵詞: electrospray, cone-jet pattern, microcapsule
相關次數: 點閱:99下載:1
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  • 本研究以微機電技術於晶片上製作管道圖形,並使用聚二甲基矽氧烷翻模技術製作晶片。利用該晶片成功產生電噴灑技術的四種基本模態,分別為滴落模態、脈衝模態、錐狀噴流模態及多噴流模態,其中在錐狀噴流模態下所產生的微膠囊粒子最為理想。
    使用三種不同溶液進行實驗,其中以包含去離子水、P123、薑黃素及海藻膠的溶液在電場強度為2.50-3.75 kV/cm與流體流率為0.3-0.7 mL/h的範圍裡,可界定出錐狀噴流模態的工作區域。由實驗結果顯示,電導率較大的溶液會使得錐狀噴流模態的工作區間往流率較小處移動,卻也相對壓縮了錐狀噴流模態的工作區域。
    當電場強度為2.9 kV/cm時,流率從0.7 mL/h降至0.3 mL/h,會使得微膠囊粒子尺寸從30-40 μm轉變為2-3 μm;當固定工作流率在0.3 mL/h時,電場強度從3.50 kV/cm降至2.90 kV/cm時,微膠囊粒子尺寸仍維持在2-4 μm。由此可知,雖然電壓及流率均為影響微膠囊之參數,但流率影響相對於電壓大。

    In this study, we present a poly (dimethylsiloxane, PDMS) planar microfluidic chip electrospray technique that is robust and extremely simple to create and operate. Three different solutions are adopted in our electrospray experiments. Four modes, namely, dripping, pulsating, cone-jet and multi-jet are observed in our experiments. According to the testing results, the cone-jet mode provides the best microcapsule quality.
    The operating condition for the cone-jet mode is identified. For the solution including DI water, P123, curcumin and alginate, the electric field intensity condition ranging from 2.50 to 3.75 kV/cm and the flow rate from 0.3 to 0.7 mL/h, we find the existence region for of the cone-jet mode. Our results show that, the cone-jet mode can be obtained in lower flow rate condition when the fluid solution has a larger electric conductivity.
    When the electric field intensity is fixed at 2.90 kV/cm and the flow rate is decreased from 0.7 to 0.3 mL/h, the microcapsules size (diameter) can be reduced from 30-40μm to 2-3 μm. Meanwhile, when the flow rate is fixed at 0.3 mL/h and the electric field intensity is decreased from 3.5 to 2.9 kV/cm, more microcapsules number with size of 2-4 μm can be produced. The results also addressed that, the flow rate provides more dorminant effect to determine the microcapsules size than the effect by changing the operating voltage.

    中文摘要 II Abstract III 誌謝 IV 目錄 VI 表目錄 VIII 圖目錄 IX 第1章 緒論 1 1.1. 前言 1 1.2. 文獻回顧 2 1.2.1. 微機電系統 3 1.2.2. 電噴灑發展史 3 1.2.3. 液滴包覆 9 1.2.4. 薑黃素 13 1.2.5. 藥物載體 15 1.2.6. 藥物傳輸系統 19 1.3. 研究動機 22 第2章 基礎理論 23 2.1. 電噴灑中離子形成之機制 23 2.2. 電噴灑影響參數 24 第3章 材料與方法 29 3.1. 光罩製作 29 3.2. 晶圓製作 30 3.2.1. 矽晶圓清洗 32 3.2.2. 光阻旋轉塗佈 33 3.2.3. 軟烘烤 34 3.2.4. 曝光 34 3.2.5. 曝光後烘烤 35 3.2.6. 顯影 35 3.2.7. 硬烘烤 35 3.3. 晶片製作 37 3.4. 氧電漿表面改質 39 3.5. 晶片設計 39 3.6. 實驗設置 41 3.6.1. 高壓電源供應器 41 3.6.2. 高速影像擷取器 42 3.6.3. 顯微鏡組 42 3.6.4. 影像分析軟體 42 3.6.5. 光學顯微鏡 43 3.6.6. 注射式泵浦 43 3.6.7. 藥品與溶液 44 第4章 實驗結果與討論 47 4.1. 電噴灑反應模態 47 4.2. 錐狀噴流模態 52 4.3. 粒徑探討 56 第5章 結果與未來展望 62 參考文獻 64 自述 70

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