本研究是利用幾丁聚醣乳酸水溶液與三聚磷酸鈉，以物理膠聯的方式固定單胞菌後，再進行酚代謝的測試。

　　從實驗的結果，我們有以下的結論：
(1)惡臭假單胞菌在被酚馴化之後對於酚有很好的適應性
(2)惡臭假單胞菌可以在固定化的過程中存活，藉由與幾丁聚醣乳酸水溶液和三聚磷酸鈉的抗菌測試中得知
(3)我們藉由低溫來增加幾丁聚醣的內聚力作用，進而減少高分子在膠體的固定化的用量
(4)固定高菌量的惡臭假單胞菌以及膠聯高濃度的膠聯劑可以在較少的天數將酚代謝

　　在酚代謝的實驗中，固定三倍菌量的惡臭假單胞菌以及膠聯1 wt%的三聚磷酸鈉所形成的幾丁聚醣顆粒有最高的代謝效率。這可能是殘留的菌液、因震盪而脫落的菌以及固定化顆粒表面上的菌所共同造成的貢獻。

　　In this study, we used chitosan lactic acid solution (CLS) and sodium tripolyphosphate (TPP) to immobilize the Pseudomonas putida by physical iontropic gelation and regarded it as a biocatalyst for the biodegradation of phenol.

　　From the results, our conclusions are as followings：
(1)The Pseudomonas putida can be acclimatized by the phenol and have well adaptability.
(2)The Pseudomonas putida stays alive in the process of immobilization according to the experimental results of the extended direct contact with CLS and TPP.
(3)Owing to the effect of the low temperature, the concentration of chitosan could be reduced in the immobilization process.
(4)A greater amount of immobilized Pseudomonas putida and/or a higher concentration of gelling agent can lead to a faster phenol degradation rate.

　　In this study, the immobilized chitosan beads embed by 3×10^9 CFU of the bacterials and gelated with 1 wt% of tripolyphosphate have the highest phenol degradation efficiency. This can be attributed to the residual cells from the washing, the cells fallen from the external surface of the beads due to vibration and the cells immobilized onto the chitosan beads.

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