硼在自然界存在 10B 和 11B 兩種天然穩定同位素，兩者的豐度分別是 19.9 % 和 80.1 %，兩個穩定同位素之間相對大的質量差異，會導致自然界中存在顯著的硼同位素分化。由於硼在天然中的兩種穩定同位素之間的相對質量差和組成變化大，因此可作為良好的古環境示蹤劑。蒸發岩和滷水的硼同位素組成也已被廣泛用於追踪古鹽度以及區分和表徵海洋和非海洋沉積環境。
在春季(3 月)和夏季(5 月)於四草安順鹽場採集各個蒸發池中逐步濃縮的滷水及 鹽樣品，以感應耦合電漿光學發射光譜儀、離子層析儀分析其元素組成，並以微昇華純化分離樣品的硼，使用多接收器感應耦合電漿質譜儀進行同位素分析。結果顯示岩鹽中流體液包體對岩鹽的微量元素濃度有顯著的影響，對硼同位素的影響不顯著，然而在蒸發過程中液體表面的岩鹽結晶，俗稱鹽花，對於硼同位素的影響。
根據研究結果，顯示流體液包體對岩鹽的元素濃度有可見的影響，流體液包體含量越多，就能給予岩鹽越多滷水原液中富含的各種微量元素。且本研究認為結晶池滷水表層結晶的鹽花形成了一層鹽殼，導致太陽的輻射熱沒有良好的傳遞到底部的滷水，熱集中在這層鹽殼上，使表面有著最強烈的蒸發作用，讓鹽花樣品擁有比較重的 δ11B 值。

Boron exists in nature as two stable isotopes, 10B and 11B, with abundances of 19.9 % and 80.1 %, respectively. The significant mass difference between the two stable isotopes leads to notable isotopic fractionation in nature. The relative mass difference and compositional variation range of the two stable boron isotopes in natural systems make them excellent tracers for paleoenvironmental studies. The isotopic compositions of boron in evaporite and brines have been widely used to track paleosalinity and to differentiate and characterize marine and non-marine sedimentary environments.
In this study, brine and salt samples were collected from progressively concentrated ponds in the Sicao Anshun Salt Field in both spring (March) and summer (May). The elemental compositions of the samples were analyzed using inductively coupled plasma optical emission spectroscopy (ICP-OES) and ion chromatography (IC), and the boron in the samples was purified by micro-sublimation and analyzed by multicollector inductively coupled plasma mass spectrometer (MC-ICP-MS). The results show that fluid inclusions have a significant influence on the trace element concentrations in salt, but the effect on the boron isotopes is not significant. However, the salt crystals on the surface of the brine during the evaporation process, known as salt flor de sal, have a strong influence on the boron isotopes.
According to obtained results, it is evident that the fluid inclusion has a visible impact on the element concentration of halite. The higher the content of fluid inclusion, the more trace elements rich in the brine solution can be provided to halite. Moreover, this study suggests that the formation of a salt crust in the form of halite crystals on the surface of brine in the crystallization pond causes the solar radiation heat to be poorly transmitted to the bottom brine. The heat is concentrated on this salt crust, resulting in the strongest evaporation on the surface, and making the halite samples have relatively heavy δ11B values.

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