氫氧基磷灰石(Hydroxyapatite, HA)為廣泛使用的生物活性材料，其所含的化學元素亦可由其它離子所取代以促進不同功能。而鍶離子的置換近年來被廣泛研究，由於鍶具有鈣不可取代的特性，且有助於骨整合及防止骨吸收。電解沉積為施加一電場，使解離後離子析出於基材表面的低成本且一步法製成。本篇研究以電解沉積法，將含鍶、鈣及磷等離子之電解液沉積於鈦基材上，形成鍶、鈣及磷化合物鍍層。
本研究主要分為三部分，分別為電解參數對鍍層的探討、鍍層材料性質分析及鍍層體外試驗分析等。電解參數結果顯示，反應溫度主要影響鍍層結晶性；電流密度大小影響鍍層均勻性；而反應時間則影響鍍層厚度。
當選定適當電解參數時，可沉積表面、厚度均勻且高結晶、高純度之鍍層。經SEM高倍觀察表面特徵，發現鍶的添加可使鍍層結構緻密化，且由原本的片狀結構轉變為針狀結構。經能量散佈光譜儀(EDS)及感應偶和電漿放射光譜儀ICP-AES亦確實發現鍶的存在，且鍍層鍶含量與電解液之鍶濃度明顯呈正比關係，其鍶離子置換率達94.5%，鍶、鈣與磷的比例亦維持1.66-1.69之間。X光繞射儀(XRD)分析相組成除鈦基材外另含有高純度鍶置換氫氧基磷灰石(Strontium substituted hydroxyapatite, Sr-HA)且隨鍶濃度提升亦增加其結晶度；經熱處理後之鍍層除基材氧化外亦無其它雜相。以上實驗均證實不同鍶濃度確實存於鍍層中，且取代鈣離子形成高純度、高結晶相之鍶置換氫氧基磷灰石。而大氣熱處理之燒結不僅可提升鍍層之結晶性亦能加強其與基材之附著力約兩倍。
經由體外試驗之浸泡模擬體液結果可知，鍍層可誘發體液中Ca32+及PO43-析出apatite結構，而經過熱處理之試片，則會降低鍶離子的釋出。由
MC3T3-E1細胞初期固定結果顯示於3小時，含鍶且熱處理之鍍層細胞已完全攤平；而未添加鍶含量均無此現象；而由免疫螢光染色亦證實含鍶且熱處理之鍍層，不僅能加速細胞初期的貼附﹐更能提升細胞初期的增生能力。顯示經過熱處理之Sr-HA其生物活性>未經熱處理之Sr-HA>HA。
本實驗成功利用電解沉積法製備低濃度之鍶置換氫氧基磷灰石，其塗佈、厚度均勻，且晶質無雜項，透過熱處理燒結後亦能提升原本Sr-HA之生物活性，期望可應用於未來人工牙根製作及改植上。

Hydroxyapatite (HA) is widely used as bioactive coating material and easily incorporated a variety of substituents in the apatite structure. The substitution of strontium (Sr) ions has been widely researched in recent years. Because strontium has irreplaceable characteristics , and promotes osseointegration and reduces bone resorption. The electrolytic deposition technique is a simple, easy controlled, low cost and one-step process method. The application of an electric field can deposit inorganic irons on the conductive substrate surface. In this study, the strontium-substituted hydroxyapatite (Sr-HA) is electrochemically deposited onto the titanium (Ti) substrate using a modulated electrical potential and electrolyte composition.
It is divided into three parts in this study, the investigation of electrolytic deposition parameters, the coating analysis of material properties , and the coating analysis of the in vitro test, respectively. In part one, it shows that the reaction temperature and the phase of coating are related ; the current density and the uniform of coating are related ; also the reaction time and the thickness of coating are related.
In part two, it indicates that suitable parameters in part one can deposite at various strontium concentration coatings with uniform, high crystallization and purity.The SEM results indicated that the flat structure of coating was transferred to needle-like and dense structure with raising strontium content. Notably, the corresponding strontium signal was detected by the EDS and ICP-AES. The rate of Sr substitution was 94.5% and the rate of (Ca+Sr)/P was between 1.6 and 1.69. The XRD analysis indicated that the phase of coating was Sr-HA and Ti. These results confirmed that strontium, calcium and phosphate were coated onto the titanium surface and major composed by Sr-HA. After heat treatment at 700oC for one hour, the crystallization of HA increased. Scratch test showed that the critical load is two times greater than one without heat treatment.
The immersion test showed that the coating can absorb Ca32+ and PO43- to form an apatite onto surface. However, the coating could restrain the release of Sr after heat treatment. The immunofluorescence test and cell observation showed that the initial unmbers and adherence in MC3T3-E1 cells with heat treatment are greater than ones without heat treatment , and they become better with the gain of Sr concentration.
In this study, it can produce the various concentration of Sr-HA coatings onto the Ti substrate with high crystallization and purity using the electrolytic deposition method. After heat treatment, it can enhance the bioactive and the strength of interface.
Therefore, it is expected to be applied to manufacture and modification of dental implant in the future.

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