本研究主要探討鹽水溪及二仁溪臭味物質的種類及其特性，利用感官分析及化學分析法對河川水樣進行臭味種類、強度、臭味物質以及水質的關係進行探討。研究中首先建立常見臭味物質之分析技術，包括使用嗅覺層次分析法(Flavor Profile Analysis, FPA)對水樣的臭味種類及強度進行初步的鑑定，以及強制選擇分析法找出主要臭味物質之嗅覺閾值，再利用氣相質譜儀(Gas Chromatograph and Mass Spectrometry Detector, GC/MSD)搭配固相微萃取法(Solid Phase Microextration, SPME) 前處理的對臭味物質做更進一步的定性與定量，以及對於臭味物質之相對臭味強度做分析。

研究中選定鹽水溪的安順排水以及竹園橋作為畜牧廢水代表採樣點，鹽水溪的北辰橋以及二仁溪的灣裡路211巷跨灣裡排水橋為生活污水的採樣點。在四次的採樣分析結果中顯示，河川中生活污水代表點最常存在的味道為臭水溝味，強度約介於5到8之間，其次為氨水味，強度介於3到6間；畜牧污水的採樣點常見的味道為糞便味/臭水溝味，強度約為6-12，顯示生活污水及畜牧污水進入河川水體中均可能會造成水中臭水溝味/糞便味的產生。臭味物質DMS及CS2、及Indole均常出現於水體中，其中Indole的出現通常伴隨著水中糞便味的產生，且Indole和大腸桿菌群及還原性硫化物具有高度正相關，和懸浮固體呈正相關；根據文獻推論當Indole存在時水中有受到糞便的污染。此外，研究中亦發現麝香味物質HHCB可以做為家庭污水污染之指標化合物。

This study was aimed to characterize the odour types, intensities and compounds associated with livestock wastewater and sewage present in Yenshui and Erren Rivers in South Taiwan. Two sensory methods, including flavor profile analysis (FPA) and sensory gas chromatograph (SGC), and a instrumental method, solid phase microextration (SPME) coupled with gas chromatograph and mass spectrometry detector(GC/MSD), were integrated to identify and quantify odour types and odourants. Four sampling points in Yenshui and Erren Rivers were chosen to represent river water polluted by sewage and livestock wastewater, including Ba-Chen in Yenshui River and Wan-Li in Erren River for Sewage, and Ann-Shun in Yenshui River and Chu-Yen in Erren River for livestock wastewater.
The FPA results reveals that the major odour type present in sewage polluted river water was sewage odour, with intensity 5-8, and the second odor type was ammonia odour, with intensity 3-6. For livestock wastewater polluted river water, the major odour type was sewage/fecal odour and the intensity was 6-12. The results suggest that sewage and fecal odour may be a good indicator to justify if a river is polluted by livestock wastewater and sewage.
Three odourants, including DMS, CS2, and indole, were commonly observed in the water samples. Among the three chemicals, indole often co-occurred with fecal odour in the same samples. In addition, indole concentrations well correlate with E. Coli. concentrations, further suggesting that the chemical is a good indicator of fecal pollution. One artificial musk chemical commonly added into detergents, HHCB, was also found to be a potential indicator of water pollution from for domestic wastewater.

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