本研究提供非侵入性的斑馬魚幼魚Danio rerio微流體觀測裝置，針對研究斑馬魚幼魚心血管與胸鰭擺動設計出相應的微流體觀測裝置。食用色素常常作食品，藥品和化妝品中的著色劑，根據研究報告指出它在生物體內具有一定的致癌和致突變作用。斑馬魚作為食用色素毒性測試的生物模型，將其胚胎暴露於胭脂紅色色素的溶液（0、0.02 ‰、0.2 ‰）與燦爛藍色色素的溶液（0、0.02 ‰、0.2 ‰），從胚胎階段到孵化和發育，並設定三個觀測時間點 (3 d.p.f.、6 d.p.f.、9 d.p.f.)。研究數據顯示，暴露於燦爛藍色素0.2‰幼魚的平均BPM (beats per second) 比控制組出高10％; 暴露於胭脂紅色素0.2‰幼魚的平均BPM高於控制組32％。進而評估幼魚的心血管參數，暴露於胭脂紅色素的幼魚與控制組並未顯示心搏排血量和心輸出量有顯著的差異，於是更廣泛研究食用色素對幼魚的影響層面。特別的是，胭脂紅色素皆對幼魚的泳動行為以及幼魚胸鰭擺動能力產生影響。濃度為0.2‰的胭脂紅色素在幼魚階段不僅顯著影響BPM，更降低幼魚的活動力與爆發力，甚至影響幼魚胸鰭擺動的能力。研究中所使用非侵入性微流體裝置提供良好的觀測條件，改善當前對小型動物模型毒性測試所遇困境，並且使用流場可視化技術對胸鰭擺動時之流場進行分析。未來微流體觀測裝置可以進一步應用研究斑馬魚幼魚的其他藥物毒性測試或泳動行為。

The present study discusses zebrafish as a biological model for toxicity testing of food coloring and further evaluating the determinant effects of this food coloring exposure on the cardiac and behavioral parameters on the model organism has been investigated in a broader manner within a non-invasive microfluidic platform. In addition, the study highlights the design and development of two novel non-invasive microfluidic devices for fixing and observation of zebrafish within the microfluidic channel. This microfluidic technology varies from the conventional approach i.e. used in the past for monitoring zebrafish. This approach avoids the excessive use of auxiliary fluids and anesthetics, which in return cause the zebrafish to suffer. The first microfluidic device was used to test the toxicity of food coloring to evaluate the effects of different food coloring concentrations on the cardiovascular functionality of the zebrafish. Due to abnormal cardiovascular function, it will change other physiological functions of zebrafish. In this work, the adverse effects of food additives on the cardiovascular functionality of the zebrafish were enlighted. The average heartbeats with the 0.2‰ concentration of the Brilliant Blue FCF additives were 10% higher than that of the control group. Consecutively, the average heartbeats with the 0.2‰ concentration of the Cochineal Red additives were 32% higher than that of the control group. Regardless of food additives, the average heartbeats in the higher concentration group were 10% higher than those in the lower concentration group. The discussion of these findings have been further extended to investigate comprehensive hydrodynamic analysis of pectoral fins of zebrafish. In spite of the fact that the additional tests are in the pipeline, it is as of now obscure how food additives affect zebrafish. Still, the deliberate outcomes displayed in this examination provide a new look in terms of using the microfluidics for a combined biological and mechanical test for biosamples.

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