在膀胱癌腫瘤發展的初期階段檢測膀胱癌標靶檢測物對於有效診斷和治療相當重要，目前膀胱癌的檢測方法仍以尿液細胞檢測和膀胱鏡檢查為主，靈敏度低、侵入式和價格昂貴的缺點使人們不願檢查導致就醫延後，而表面增強拉曼散射(surface enhanced Raman scattering, SERS)其高靈敏度、非侵入性、高辨識度、即時檢測的特性可以解決這些難題，本研究透過奈米壓印技術和光還原法製作SERS基板，提出以SERS技術的免疫檢測法(SERS-based immunoassay)檢測由美國食品藥物管理局(FDA)公布的膀胱癌標靶檢測物Complement Factor H-related protein(CFH)。在羅丹明6G(Rhodamine 6G, R6G)和孔雀綠(Malachite green, MG)的探測極限分別達到10-8 M和10-10 M，在MG的部分甚至低於歐盟的標準(5.48 x 10-9 M)，SERS感測器的均勻性和重複性，其RSD分別是8.79%和8.93%，而CFH的濃度測試只能從10-5 g/ml到10-7 g/ml並且只成功過一次。本研究的SERS基板不僅製程成本便宜、時間快速，更能大量製造，也有良好的均勻性和重複性，為膀胱癌患者提供更舒適、更便利、更便宜的檢測方式。

In the early stage of the bladder cancer, target detection of bladder cancer is very important for effective diagnosis and treatment. Until now, cystoscopy and urine cytology are still the mainly detection method for bladder cancer. However, the low sensitivity, invasive detection and expensive price make people don’t want to diagnosis. Surface enhanced Raman scattering (SERS) which has high sensitivity, high identify, noninvasive detection and point of acre can resolve these problems. In our research, we make the SERS substrate through the nanoimprint and photoreduction then apply to the SERS-based immunoassay to detect the Complement Factor H-related protein (CFH), the detection target of bladder cancer, which is approved by U.S. Food and Drug Administration (FDA). In molecule measurement, the detection limit of rhodamine 6G (R6G) and malachite green (MG) is 10-8 M and 10-10 M, respectively. The concentration of MG even lower than the standard of European Union (5.48 x 10-9 M). The uniformity and reproducibility of SERS substrate are great, the relative standard deviation (RSD) is 8.79% and 8.93%, respectively. The CFH test only detect from10-5 g/ml to 10-7 g/ml and success once. The SERS substrate we make not only simple and low cost but also has high uniformity and reproducibility. We provide the more comfortable, more convenient, and cheaper detection method for the bladder cancer patient.

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