近年來發展並採用植入式的人工牙根日益普及，金屬鈦以及其合金擁有良好的化學穩定性與生物相容性，廣泛被應用於骨科與牙科領域，然而於臨床上仍被視為生物惰性，因此如何進行有效材料改質，並模擬於生物體內的實際情形，改善生物活性進而提升骨整合是研究的主要關鍵。
　　本實驗利用噴砂酸蝕（SLA）的技術，使鈦表面同時具有微米與次微米等級的孔洞結構，並運用前處理控制粗糙度。接著配合微弧氧化技術（MAO）使表面具有孔洞形態的氧化層，並透過製備含不同鍶濃度（Sr）來改變其化學組成。人體內的氧濃度相較於正常大氣下是低氧含量，因此生物實驗設計在低氧（hypoxia）環境下進行，細胞培養分別控制在不同氧濃度下進行實驗。
　　由掃描式電子顯微鏡觀察SLA 、MAO表面呈現均勻結構，粗糙儀測得由噴沙壓力控制順利產生不同粗糙度的SLA表面，化學組成與晶體結構分別利用EDS與XRD測試MAO與 SrMAO表面，在不同低氧濃度下培養細胞，進行生物毒性測試（MTT）、生物活性測試（ALP）。結果顯示在經過SLA處理的表面型態、SrMAO含鍶的氧化層組成,，並且在低氧的環境下均呈現良好的生物反應。

There is a growing popularity of dental implants in the market. Titanium (Ti) and its alloys are among the most successful implantable materials for dental and orthopedic applications due to the properties of good chemical stability and biocompatibility. Still, they are considered to be bio-inert materials in clinical.
In this study, cell culture study performed under 1%, 5%, and 10% hypoxic stress simulating the condition in the human body to compare with that under normal atmospheric oxygen tension of 21%. First, the Ti discs were prepared to increase and control roughness by different pressure of sandblasting (SB). Combined acid-etching (SLA) to obtain both micron and sub-micron structure on the surface. Then, the micro-arc oxidation (MAO) technique was used to improve the characteristics on Ti surface. MAO coating formed in the electrolytes with different strontium (Sr) content. Pre-osteoblastic cells MC3T3-E1 was chosen for cell behavior evaluation in vitro.
From the observation of scanning electron microscope (SEM) images, all modified surfaces had uniform porous structure, including SLA, MAO, and SrMAO. Surface analyzer showed that SB pressure can control the roughness of SLA well. Used energy dispersive X-ray spectrometer (EDS) for chemical composition and different Sr content was detected in the oxide coating of MAO. Thin film X-ray diffraction (TF-XRD) results indicated the phase of MAO coating was anatase and rutile. Cell culture experiments demonstrated that surface modification and Sr content will improve the growth of MC3T3-E1, especially in hypoxia condition with significant differences.

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